[嗜肺军团菌细胞溶素的特性]

[Characteristics of the cytolysin of Legionella pneumophila].

作者信息

Belyĭ Iu F, Vertiev Iu V, Tartakovskiĭ I S, Ezepchuk Iu V, Prozorovskiĭ S V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1988 Feb(2):4-7.

Abstract

A thermolabile cytolysin was purified from liquid culture of L. pneumophila. Its homogenicity was determined by the Ouchterlony double immunodiffusion and SDS-electrophoresis in polyacrylamide gel. The molecular weight of cytolysin was ca. 37 kDa. Analysis of amino acid composition revealed a high proportion of aromatic, dicarbonic amino acids, and methionine. The minimal cytolytic concentration for CHO cells and erythrocytes was ca. 1 microgram/ml: Purified cytolysin in doses of 10-60 micrograms caused haemorrhage and necrosis when injected i. c. into guinea pigs.

摘要

从嗜肺军团菌的液体培养物中纯化出一种热不稳定细胞溶素。通过免疫双扩散和聚丙烯酰胺凝胶中的SDS电泳测定其纯度。细胞溶素的分子量约为37 kDa。氨基酸组成分析显示芳香族、二羧基氨基酸和甲硫氨酸的比例很高。对CHO细胞和红细胞的最小细胞溶解浓度约为1微克/毫升：以10 - 60微克剂量纯化的细胞溶素经脑内注射到豚鼠体内会引起出血和坏死。