Horuztepe Sidika Aynur, Ergin Esra, Onen Alev, Gürgan Sevil
Private practice in Ankara, Turkey.
Hacettepe University, School of Dentistry, Department of Restorative Dentistry, Ankara, Turkey.
Acta Stomatol Croat. 2020 Dec;54(4):382-391. doi: 10.15644/asc54/4/5.
To compare the subsurface mineral loss preventing capability of resin infiltration technique with topical fluoride and fissure sealant applications to demineralized occlusal fissures under simulated oral conditions.
Occlusal surfaces of 64 extracted intact human third molars were demineralized. Next, the teeth were classified into four groups according to preventive applications (n = 16): G1, Specimens used as the control group with no preventive treatment; G2, Topical fluoride application (APF Gel/ DEEPAK); G3, Fissure sealant application (ClinproTMSealant/ 3M ESPE); and G4, Resin infiltration technique (Icon/ DMG). Chemical compositions before pH cycling were evaluated for eight specimens from each group. The remaining eight teeth from each group were subjected to pH cycling for 15 days to simulate the oral conditions. Subsequently, the specimens were fractured after immersion in liquid nitrogen and the subsurface fluoride (F), calcium (Ca), phosphorus (P) levels, and Ca/P ratio of each specimen were measured using energy dispersive x-ray spectrometer (EDS). The data were subjected to statistical analysis (p = 0.05). The effects of preventive applications to surface topography of specimens were evaluated using scanning electron microscope (SEM).
There were no significant differences among the groups in subsurface F, Ca, and P levels and Ca/P ratios before or after pH cycling (p > 0.05). All three preventive applications were effective during pH cycling according to SEM observations.
The subsurface mineral loss preventing capability of resin infiltration technique applied to occlusal fissures was comparable to topical fluoride and fissure sealant applications.
The resin infiltration technique could represent a valid alternative to traditionally used both preventive and restorative treatments for treating initial carious lesions on occlusal fissures, offering the advantages of better resin penetration and retention.
在模拟口腔条件下，比较树脂渗透技术与局部用氟化物及窝沟封闭剂对脱矿咬合面窝沟的预防牙本质深层矿质流失的能力。
选取64颗完整拔除的人第三磨牙的咬合面进行脱矿处理。接下来，根据预防处理方法将牙齿分为四组（每组n = 16）：G1组，作为对照组，不进行预防处理；G2组，局部应用氟化物（APF凝胶/迪帕克）；G3组，应用窝沟封闭剂（ClinproTM封闭剂/3M ESPE）；G4组，采用树脂渗透技术（Icon/DMG）。对每组8个样本在pH循环处理前的化学成分进行评估。每组剩余的8颗牙齿进行15天的pH循环处理以模拟口腔条件。随后，将样本浸入液氮中使其断裂，使用能量色散X射线光谱仪（EDS）测量每个样本的牙本质深层氟（F）、钙（Ca）、磷（P）水平以及Ca/P比值。对数据进行统计分析（p = 0.05）。使用扫描电子显微镜（SEM）评估预防处理对样本表面形貌的影响。
在pH循环处理前后，各组之间牙本质深层的F、Ca、P水平以及Ca/P比值均无显著差异（p > 0.05）。根据SEM观察，所有三种预防处理在pH循环处理期间均有效。
应用于咬合面窝沟的树脂渗透技术预防牙本质深层矿质流失的能力与局部用氟化物及窝沟封闭剂相当。
树脂渗透技术可为治疗咬合面窝沟处的早期龋损提供一种有效的替代传统预防性和修复性治疗的方法，具有更好的树脂渗透和保留优势。
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