Chen Jia-Min, Wang Tao, Guo Qiao-Sheng, Li Hao-Wen, Zuo Lin, Zou Qing-Jun, Yang Feng
Institute of Chinese Medicinal Materials,Nanjing Agricultural University Nanjing 210095,China.
Zhongguo Zhong Yao Za Zhi. 2021 Feb;46(4):907-914. doi: 10.19540/j.cnki.cjcmm.20201122.102.
The study is aiming at investigating the application of entropy weight TOPSIS method in the comparison of the scavenging effect of DPPH, ABTS and hydroxyl radical and the inhibition effect of xanthine oxidase(XOD) and lipoxygenase(LOX) of Chrysanthemum indicum. The DPPH, ABTS, salicylic acid and spectrophotometry were used to determine the scavenging effect of DPPH, ABTS and hydroxyl radical and the inhibition effect of xanthine oxidase(XOD) and lipoxygenase(LOX) of Ch. indicum from 31 different areas in vitro. Take the half inhibition rate of as the evaluation index, two principal components were extracted by the principal component analysis, and their cumulative contribution rate reached at 92.4%. The different areas of Ch. indicum could be divided into Dabei Mountain and Qinling-Taihang Mountain by use principal component to analysis. The entropy weight TOPSIS method was used to objectively assign weights to five indexes, calculate the weight of each index and set up the best and worst scheme of the evaluation object, and the relative proximity(C_i) was used as the measure to construct the multi-index comprehensive evaluation model of Ch. indicum. And then sort with the relative proximity value. The results showed that the relative proximity was between 0.098 and 0.983 which represents there were significant differences in the scavenging effect of DPPH, ABTS and hydroxyl radical and the inhibition effect of xanthine oxidase(XOD) and lipoxygenase(LOX) between extracts of Ch. indicum from different areas. The Ch. indicum from Dabie Mountain area have a relatively high relative degree of measurement and high-quality ranking. Taken together, the quality of Ch. indicum.from the Dabie Mountain area is better. The index weight coefficient and the classification result of producing area are basically consistent with the result of principal component analysis. The results show that the TOPSIS method based on entropy weight method can be used to evaluate the comprehensive quality of Ch. indicum.
本研究旨在探讨熵权TOPSIS法在比较野菊花对DPPH、ABTS和羟自由基的清除作用以及对黄嘌呤氧化酶（XOD）和脂氧合酶（LOX）的抑制作用中的应用。采用DPPH法、ABTS法、水杨酸法和分光光度法，体外测定了31个不同产地野菊花对DPPH、ABTS和羟自由基的清除作用以及对黄嘌呤氧化酶（XOD）和脂氧合酶（LOX）的抑制作用。以半数抑制率为评价指标，通过主成分分析提取两个主成分，其累计贡献率达到92.4%。利用主成分分析法可将不同产地的野菊花分为大别山和秦岭-太行山两类。采用熵权TOPSIS法对5项指标进行客观赋权，计算各指标权重，建立评价对象的最优和最劣方案，并以相对贴近度（Ci）为测度构建野菊花多指标综合评价模型。然后根据相对贴近度值进行排序。结果表明，相对贴近度在0.098～0.983之间，表明不同产地野菊花提取物对DPPH、ABTS和羟自由基的清除作用以及对黄嘌呤氧化酶（XOD）和脂氧合酶（LOX）的抑制作用存在显著差异。大别山区野菊花的相对测度度较高，质量排序靠前。综合来看，大别山区野菊花的品质较好。指标权重系数和产地分类结果与主成分分析结果基本一致。结果表明，基于熵权法的TOPSIS法可用于评价野菊花的综合品质。
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