乙型肝炎病毒 X 蛋白调节肝癌中趋化因子 CCL15 的上调。
Hepatitis B Virus X Protein Modulates Chemokine CCL15 Upregulation in Hepatocellular Carcinoma.
机构信息
The Third Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.
Department of Clinical Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.
出版信息
Anticancer Agents Med Chem. 2021 Oct 28;21(16):2198-2203. doi: 10.2174/1871520621666210302083407.
BACKGROUND
Hepatitis B virus X protein (HBx) is an indispensable progression factor in Hepatocellular Carcinoma (HCC). CCL15 could be a peculiar proteomic biomarker of HCC with tumorigenesis and tumor invasion.
OBJECTIVE
The aim of the study was to explore the relationship between HBx and CCL15 expression in HCC.
METHODS
HBV-positive HCC pathological tissue samples and corresponding adjacent non-tumor liver tissues were collected. The expression of HBx and CCL15 was analyzed by immunohistochemistry, real-time Polymerase Chain Reaction (PCR), and western blot analysis in tissues or in vitro.
RESULTS
The levels of CCL15 mRNA and protein expression in HCC samples were observably higher than those of adjacent non-tumor liver tissues. The CCL15 was significantly associated with the expression of HBx in HBV-positive HCC samples. The up-regulation of HBx induced CCL15 expression in vitro. The high expression score of CCL15 was significant associated with the poor prognosis of HCC patients.
CONCLUSION
The CCL15 expression was observably associated with HBx in HCC patients. The CCL15 may be considered as an indicator in the clinical management of HBV-associated HCC.
背景
乙型肝炎病毒 X 蛋白（HBx）是肝细胞癌（HCC）中不可或缺的进展因子。趋化因子配体 15（CCL15）可能是 HCC 具有致癌和肿瘤侵袭性的独特蛋白质组生物标志物。
目的
本研究旨在探讨 HBx 与 HCC 中 CCL15 表达之间的关系。
方法
收集 HBV 阳性 HCC 病理组织样本及相应的相邻非肿瘤肝组织。采用免疫组织化学、实时聚合酶链反应（PCR）和 Western blot 分析组织或体外 HBx 和 CCL15 的表达。
结果
与相邻非肿瘤肝组织相比，HCC 样本中 CCL15mRNA 和蛋白表达水平明显升高。CCL15 在 HBV 阳性 HCC 样本中与 HBx 的表达显著相关。HBx 的上调诱导了体外 CCL15 的表达。CCL15 的高表达评分与 HCC 患者的不良预后显著相关。
结论
CCL15 在 HCC 患者中与 HBx 明显相关。CCL15 可作为 HBV 相关 HCC 临床管理的指标。
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