Department of Chemistry "Ugo Schiff" and CSGI, University of Florence, Via Della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.
J Nanosci Nanotechnol. 2021 May 1;21(5):2872-2878. doi: 10.1166/jnn.2021.19049.
The recently-discovered endogenous formation of amorphous magnesium-calcium phosphate nanoparticles (AMCPs) in human distal small intestine occurs in a complex environment, which is rich in biologically-relevant molecules and macromolecules that can shape the properties and the stability of these inorganic particles. In this work, we selected as case studies four diverse molecules, which have different properties and are representative of intestinal luminal components, namely butyric acid, lactose, gluten and peptidoglycan. We prepared AMCPs in the presence of these four additives and we investigated their effect on the features of the particles in terms of morphology, porosity, chemical nature and incorporation/adsorption. The combined use of electron microscopy, infrared spectroscopy and thermal analysis showed that while the morphology and microstructure of the particles do not depend on the type of additive present during the synthesis, AMCPs are able to incorporate a significant amount of peptidoglycan, similarly to the process in which they are involved .
在人类远端小肠中最近发现的无定形镁钙磷酸盐纳米颗粒 (AMCPs) 的内源性形成发生在复杂的环境中，该环境富含具有生物相关性的分子和大分子，这些分子和大分子可以改变这些无机颗粒的性质和稳定性。在这项工作中，我们选择了四种不同的分子作为案例研究，这些分子具有不同的性质，并且是肠腔成分的代表，即丁酸、乳糖、面筋和肽聚糖。我们在这四种添加剂的存在下制备了 AMCPs，并研究了它们对颗粒特性的影响，包括形态、孔隙率、化学性质以及掺入/吸附。电子显微镜、红外光谱和热分析的联合使用表明，尽管颗粒的形态和微观结构不取决于合成过程中存在的添加剂类型，但 AMCPs 能够掺入大量的肽聚糖，类似于它们参与的过程。
