Ruer-Laventie Julie, Jayachandran Rajesh, Schmaler Mathias, Rossi Simona W, Pieters Jean
Biozentrum, University of Basel, Basel, Switzerland.
Department of Biomedicine, University and University Hospital Basel, Basel, Switzerland.
Bio Protoc. 2020 Feb 20;10(4):e3531. doi: 10.21769/BioProtoc.3531.
Skin transplantation in mice is an important procedure to evaluate immune responses generated against heterologous grafts, especially given its highly immunogenic nature. In fact, skin is one of the most challenging organs in terms of allograft retention. In this protocol, we provide a detailed procedure for skin grafting using the tail skin as donor organ that is grafted on the dorsal site of thoracic cage in a recipient mouse. We also provide protocols for the systematic analysis of lymphoid organ analysis in transplanted mice. Together these protocols may be valuable for evaluation of parameters that affect skin grafting, including genetic factors, immune cell activation as well as the analysis of compounds that may be useful in allowing graft tolerance.
小鼠皮肤移植是评估针对异种移植物产生的免疫反应的重要程序，尤其是考虑到其高度免疫原性的特性。事实上，就同种异体移植的保留而言，皮肤是最具挑战性的器官之一。在本方案中，我们提供了一个详细的程序，使用尾部皮肤作为供体器官进行皮肤移植，移植到受体小鼠胸廓的背部位置。我们还提供了对移植小鼠淋巴器官进行系统分析的方案。这些方案一起可能对评估影响皮肤移植的参数很有价值，包括遗传因素、免疫细胞激活以及对可能有助于实现移植耐受的化合物的分析。
