内吞作用检测于…… （你提供的原文不完整，仅能翻译到这个程度）
Endocytosis Detection in .
作者信息
Liu Muxing, Zhang Zhengguang
机构信息
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
出版信息
Bio Protoc. 2019 Aug 5;9(15):e3322. doi: 10.21769/BioProtoc.3322.
Abstract
Endocytosis is an intracellular trafficking pathway that occurs in nutrient uptake, signal transduction and reconstruction of cell polarity and is conserved in eukaryotic cells. In fungi, endocytosis plays crucial roles in the physiology of hyphal growth and pathogenicity. vidence for endocytosis in filamentous fungi is detected by the membrane-selective dyes FM4-64. Cells of a range of filamentous fungal species readily take up these dyes. However, the method for endocytosis detection has not been well established in . Here, we provide a protocol for tracking endocytosis in .
摘要
内吞作用是一种细胞内运输途径，发生在营养物质摄取、信号转导以及细胞极性重建过程中，并且在真核细胞中是保守的。在真菌中，内吞作用在菌丝生长和致病性的生理过程中发挥着关键作用。丝状真菌内吞作用的证据是通过膜选择性染料FM4-64检测到的。一系列丝状真菌物种的细胞很容易摄取这些染料。然而，内吞作用检测方法在[此处原文缺失相关内容]中尚未得到很好的确立。在这里，我们提供了一种在[此处原文缺失相关内容]中追踪内吞作用的方案。
相似文献
1
Endocytosis Detection in .内吞作用检测于…… （你提供的原文不完整，仅能翻译到这个程度）
Bio Protoc. 2019 Aug 5;9(15):e3322. doi: 10.21769/BioProtoc.3322.
2
MoVam7, a conserved SNARE involved in vacuole assembly, is required for growth, endocytosis, ROS accumulation, and pathogenesis of Magnaporthe oryzae.MoVam7，一种参与液泡组装的保守 SNARE，对于稻瘟病菌的生长、内吞作用、ROS 积累和致病性是必需的。
PLoS One. 2011 Jan 24;6(1):e16439. doi: 10.1371/journal.pone.0016439.
3
Arf6 controls endocytosis and polarity during asexual development of Magnaporthe oryzae.Arf6在稻瘟病菌无性发育过程中控制内吞作用和极性。
FEMS Microbiol Lett. 2016 Nov;363(22). doi: 10.1093/femsle/fnw248. Epub 2016 Nov 2.
4
Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae.使用FM4-64进行共聚焦显微镜检查作为分析活真菌菌丝内吞作用和囊泡运输的工具。
J Microsc. 2000 Jun;198(Pt 3):246-59. doi: 10.1046/j.1365-2818.2000.00708.x.
5
Live-cell imaging of endocytosis during conidial germination in the rice blast fungus, Magnaporthe grisea.稻瘟病菌分生孢子萌发过程中内吞作用的活细胞成像
Fungal Genet Biol. 2002 Dec;37(3):233-44. doi: 10.1016/s1087-1845(02)00535-2.
6
Functional analysis of MoSnf7 in Magnaporthe oryzae.MoSnf7 在稻瘟病菌中的功能分析。
Fungal Genet Biol. 2018 Dec;121:29-45. doi: 10.1016/j.fgb.2018.09.005. Epub 2018 Sep 18.
7
Endocytosis is crucial for cell polarity and apical membrane recycling in the filamentous fungus Aspergillus oryzae.内吞作用对于丝状真菌米曲霉的细胞极性和顶端膜循环至关重要。
Eukaryot Cell. 2009 Jan;8(1):37-46. doi: 10.1128/EC.00207-08. Epub 2008 Nov 21.
8
MoCAP proteins regulated by MoArk1-mediated phosphorylation coordinate endocytosis and actin dynamics to govern development and virulence of Magnaporthe oryzae.由MoArk1介导的磷酸化作用调控的MoCAP蛋白协调内吞作用和肌动蛋白动力学，从而控制稻瘟病菌的发育和毒力。
PLoS Genet. 2017 May 25;13(5):e1006814. doi: 10.1371/journal.pgen.1006814. eCollection 2017 May.
9
The syntaxin protein (MoSyn8) mediates intracellular trafficking to regulate conidiogenesis and pathogenicity of rice blast fungus.Syntaxin蛋白（MoSyn8）介导细胞内运输以调节稻瘟病菌的分生孢子形成和致病性。
New Phytol. 2016 Mar;209(4):1655-67. doi: 10.1111/nph.13710. Epub 2015 Nov 2.
10
Two Rab5 Homologs Are Essential for the Development and Pathogenicity of the Rice Blast Fungus .两个Rab5同源物对稻瘟病菌的发育和致病性至关重要。
Front Plant Sci. 2017 May 5;8:620. doi: 10.3389/fpls.2017.00620. eCollection 2017.
引用本文的文献
1
Influence of Two Hexose Transporters on Substrate Affinity and Pathogenicity in .两种己糖转运蛋白对……中底物亲和力和致病性的影响
Microorganisms. 2024 Mar 28;12(4):681. doi: 10.3390/microorganisms12040681.
本文引用的文献
1
MoCAP proteins regulated by MoArk1-mediated phosphorylation coordinate endocytosis and actin dynamics to govern development and virulence of Magnaporthe oryzae.由MoArk1介导的磷酸化作用调控的MoCAP蛋白协调内吞作用和肌动蛋白动力学，从而控制稻瘟病菌的发育和毒力。
PLoS Genet. 2017 May 25;13(5):e1006814. doi: 10.1371/journal.pgen.1006814. eCollection 2017 May.
2
Vps1 in the late endosome-to-vacuole traffic.Vps1 在晚期内体到液泡运输中的作用。
J Biosci. 2013 Mar;38(1):73-83. doi: 10.1007/s12038-012-9295-2.
3
Functional analysis of the putative AAA ATPase AipA localizing at the endocytic sites in the filamentous fungus Aspergillus oryzae.丝状真菌米曲霉内吞部位定位的假定 AAA ATP 酶 AipA 的功能分析。
FEMS Microbiol Lett. 2011 Jul;320(1):63-71. doi: 10.1111/j.1574-6968.2011.02288.x. Epub 2011 May 9.
4
Requirements of Slm proteins for proper eisosome organization, endocytic trafficking and recycling in the yeast Saccharomyces cerevisiae.Slm 蛋白在酵母酿酒酵母中正确的内体小体组织、内吞运输和回收中的要求。
J Biosci. 2011 Mar;36(1):79-96. doi: 10.1007/s12038-011-9018-0.
5
Monitoring autophagy in yeast using FM 4-64 fluorescence.使用FM 4-64荧光监测酵母中的自噬。
Methods Enzymol. 2008;451:79-88. doi: 10.1016/S0076-6879(08)03207-2.
6
Endocytosis is crucial for cell polarity and apical membrane recycling in the filamentous fungus Aspergillus oryzae.内吞作用对于丝状真菌米曲霉的细胞极性和顶端膜循环至关重要。
Eukaryot Cell. 2009 Jan;8(1):37-46. doi: 10.1128/EC.00207-08. Epub 2008 Nov 21.
7
Tracing the endocytic pathway of Aspergillus nidulans with FM4-64.利用FM4-64追踪构巢曲霉的内吞途径。
Fungal Genet Biol. 2005 Dec;42(12):963-75. doi: 10.1016/j.fgb.2005.09.004. Epub 2005 Nov 15.
8
A pathway for association of receptors, adaptors, and actin during endocytic internalization.内吞内化过程中受体、衔接蛋白和肌动蛋白的关联途径。
Cell. 2003 Nov 14;115(4):475-87. doi: 10.1016/s0092-8674(03)00883-3.
9
Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae.使用FM4-64进行共聚焦显微镜检查作为分析活真菌菌丝内吞作用和囊泡运输的工具。
J Microsc. 2000 Jun;198(Pt 3):246-59. doi: 10.1046/j.1365-2818.2000.00708.x.
10
A putative endosomal t-SNARE links exo- and endocytosis in the phytopathogenic fungus Ustilago maydis.一种假定的内体t-SNARE连接植物致病真菌玉米黑粉菌中的胞吐作用和胞吞作用。
EMBO J. 2000 May 2;19(9):1974-86. doi: 10.1093/emboj/19.9.1974.
Zotero 插件