Liu Muxing, Zhang Zhengguang
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
Bio Protoc. 2019 Aug 5;9(15):e3322. doi: 10.21769/BioProtoc.3322.
Endocytosis is an intracellular trafficking pathway that occurs in nutrient uptake, signal transduction and reconstruction of cell polarity and is conserved in eukaryotic cells. In fungi, endocytosis plays crucial roles in the physiology of hyphal growth and pathogenicity. vidence for endocytosis in filamentous fungi is detected by the membrane-selective dyes FM4-64. Cells of a range of filamentous fungal species readily take up these dyes. However, the method for endocytosis detection has not been well established in . Here, we provide a protocol for tracking endocytosis in .
内吞作用是一种细胞内运输途径,发生在营养物质摄取、信号转导以及细胞极性重建过程中,并且在真核细胞中是保守的。在真菌中,内吞作用在菌丝生长和致病性的生理过程中发挥着关键作用。丝状真菌内吞作用的证据是通过膜选择性染料FM4-64检测到的。一系列丝状真菌物种的细胞很容易摄取这些染料。然而,内吞作用检测方法在[此处原文缺失相关内容]中尚未得到很好的确立。在这里,我们提供了一种在[此处原文缺失相关内容]中追踪内吞作用的方案。