Collaborative Innovation Center for Birth Defect Research and Transformation of Shandong Province, Jining Medical University, Jining, 272067, China.
Shandong Institute for Product Quality Inspection, Jinan, 250102, China.
World J Microbiol Biotechnol. 2021 Mar 2;37(4):58. doi: 10.1007/s11274-021-03023-4.
Blakeslea trispora, a heterothallic Zygomycota with two mating types (termed "plus" and "minus"), is an ideal source of lycopene and β-carotene. The lycopene and β-carotene yields when the two type strains are used for fermentation separately are lower than those when they are joint together. To enhance the yield of lycopene and β-carotene in B. trispora, protoplast fusion technology was carried out between ATCC 14,271 (+) and ATCC 14,272 (-). After protoplast preparation, protoplast fusion, fusion sorting, fusion regeneration, and high-throughput screening, two fusions (Fu-1and Fu-2) with high lycopene and β-carotene yields were obtained. The lycopene yields of Fu-1 and Fu-2 were increased to 0.60 mg/gDW and 0.90 mg/gDW, which were respectively 3.62- and 5.44-fold those of 14,271 and 1.76- and 2.64-fold those of 14,272. The β-carotene yields of Fu-1 and Fu-2 were increased to 22.07 mg/gDW and 36.93 mg/gDW, which were respectively 1.72- and 2.89-fold those of 14,271 and 1.23- and 2.06-fold those of 14,272. In this study, the protoplast fusion technique was successfully used in Blakeslea trispora, providing new ideas for improving lycopene and β-carotene production.
布勒氏犁头霉是一种异宗配合的接合菌，具有两种交配型（分别称为“+”和“-”），是番茄红素和β-胡萝卜素的理想来源。当使用两种类型的菌株分别进行发酵时，番茄红素和β-胡萝卜素的产量低于它们联合使用时的产量。为了提高布勒氏犁头霉中番茄红素和β-胡萝卜素的产量，我们在 ATCC 14,271（+）和 ATCC 14,272（-）之间进行了原生质体融合技术。在制备原生质体、原生质体融合、融合分选、融合再生和高通量筛选之后，获得了两个具有高番茄红素和β-胡萝卜素产量的融合体（Fu-1 和 Fu-2）。Fu-1 和 Fu-2 的番茄红素产量分别提高到 0.60mg/gDW 和 0.90mg/gDW，分别是 14,271 的 3.62 倍和 5.44 倍，是 14,272 的 1.76 倍和 2.64 倍。Fu-1 和 Fu-2 的β-胡萝卜素产量分别提高到 22.07mg/gDW 和 36.93mg/gDW，分别是 14,271 的 1.72 倍和 2.89 倍，是 14,272 的 1.23 倍和 2.06 倍。在本研究中，原生质体融合技术成功应用于布勒氏犁头霉，为提高番茄红素和β-胡萝卜素的生产提供了新的思路。
