用于评估伴有心肌舒张功能障碍的2型糖尿病大鼠代谢状态的无标记和非侵入性方法。
Label-free and noninvasive method for assessing the metabolic status in type 2 diabetic rats with myocardium diastolic dysfunction.
作者信息
Guo Kai, Wu Junxin, Kong Yawei, Zhou Li, Li Wei, Fei Yiyan, Ma Jiong, Mi Lan
机构信息
Department of Cardiology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, China.
These authors contributed equally to this work.
出版信息
Biomed Opt Express. 2020 Dec 17;12(1):480-493. doi: 10.1364/BOE.413347. eCollection 2021 Jan 1.
This study assesses the metabolic status of rat diabetic cardiomyopathy (DCM) models. Echocardiography is used to detect the diastolic dysfunction in type 2 diabetic rats, and a lower threshold for inducible atrial fibrillation is found in type 2 diabetic rats with diastolic dysfunction compared to the control. Metabolic abnormalities are detected by status changes of reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H), which is an essential coenzyme in cells or tissues. Fluorescence lifetime imaging microscopy (FLIM) is used to monitor changes in NAD(P)H in both myocardial tissues and blood. FLIM reveals that the protein-bound proportion of NAD(P)H in rat myocardium in the DCM group is smaller than the control group, which indicates the oxidative phosphorylation rate of the DCM group decreased. Similar results are found for blood plasma of DCM rats by the FLIM study. FLIM exhibits high potential for screening DCM as a label-free, sensitive, and noninvasive method.
本研究评估大鼠糖尿病性心肌病（DCM）模型的代谢状态。采用超声心动图检测2型糖尿病大鼠的舒张功能障碍，发现与对照组相比，存在舒张功能障碍的2型糖尿病大鼠诱发房颤的阈值更低。通过还原型烟酰胺腺嘌呤二核苷酸（磷酸）（NAD(P)H）的状态变化检测代谢异常，NAD(P)H是细胞或组织中的一种必需辅酶。使用荧光寿命成像显微镜（FLIM）监测心肌组织和血液中NAD(P)H的变化。FLIM显示，DCM组大鼠心肌中与蛋白质结合的NAD(P)H比例小于对照组，这表明DCM组的氧化磷酸化速率降低。通过FLIM研究，在DCM大鼠的血浆中也发现了类似结果。作为一种无标记、灵敏且无创的方法，FLIM在筛查DCM方面具有很高的潜力。
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