Yang Ning, Zhang Yue, Chen Lu, Wang Wei, Liu Ruirui, Gao Run, Zhou Yaping, Li Hui
College of Life Sciences, Northwest Normal University, Lanzhou, 730070, PR China.
Biochem Biophys Rep. 2021 Feb 12;26:100952. doi: 10.1016/j.bbrep.2021.100952. eCollection 2021 Jul.
Jasmonic acid (JA) is regarded as an endogenous regulator which plays an important role in regulating plant growth, development and stress response. Using the seedlings of ecotype Col-0 (wild-type, WT), phospholipase Dδ (PLDδ) deficient mutant (), the G protein α subunit (GPA1) deficient mutant (), 9-Lipoxygenase (9-LOX) deficient mutants ( and ) as materials, the effects of JA responding to osmotic stress and the functions of G protein and PLDδ in this response were investigated. Exogenous MeJA reduced the EL and MDA in WT, but increased the EL and MDA in and , indicating that GPA1 and PLDδ were involved in the protection of JA on the membrane. The genes expression levels, and the activities of PLDδ and LOX1 were significantly induced by osmotic stress. The LOX activity and JA content in seedings were lower obviously than those in WT, but were markedly increased and were higher than WT after applying phosphatidic acid (PA). These results demonstrated that JA responded to osmotic stress by regulating G protein and PLDδ in . PLDδ was located upstream of 9-LOX and involved in the JA biosynthesis.
茉莉酸（JA）被认为是一种内源性调节剂，在调节植物生长、发育和应激反应中发挥重要作用。以生态型Col-0（野生型，WT）、磷脂酶Dδ（PLDδ）缺陷型突变体、G蛋白α亚基（GPA1）缺陷型突变体、9-脂氧合酶（9-LOX）缺陷型突变体（和）的幼苗为材料，研究了JA对渗透胁迫的响应以及G蛋白和PLDδ在此响应中的功能。外源茉莉酸甲酯降低了WT中的电解质渗漏率（EL）和丙二醛（MDA），但增加了和中的EL和MDA，表明GPA1和PLDδ参与了JA对膜的保护作用。渗透胁迫显著诱导了基因表达水平以及PLDδ和脂氧合酶1（LOX1）的活性。幼苗中的脂氧合酶活性和JA含量明显低于WT，但在施加磷脂酸（PA）后显著增加且高于WT。这些结果表明，JA通过调节中的G蛋白和PLDδ对渗透胁迫作出响应。PLDδ位于9-LOX的上游并参与JA生物合成。
