Faculty of Biology, University of Bialystok, Ciolkowskiego 1J, 15-245 Bialystok, Poland.
Int J Mol Sci. 2021 Feb 23;22(4):2214. doi: 10.3390/ijms22042214.
Plant-pollinator interactions significantly influence reproductive success (RS) and drive the evolution of pollination syndromes. In the context of RS, mainly the role of flower morphology is touched. The importance of nectar properties is less studied, despite its significance in pollination effectiveness. Therefore, the aim of this study was to test selection on flower morphology and nectar chemistry in the generalistic orchid . In 2019-2020, we measured three floral displays and six flower traits, pollinaria removal (PR), female reproductive success (FRS), and determined the soil properties. The sugars and amino acids (AAs) were analyzed using the HPLC method. Data were analyzed using multiple statistical methods (boxplots, ternary plot, one-way ANOVA, Kruskal-Wallis test, and PCA). Variation of flower structure and nectar chemistry and their weak correlation with RS confirms the generalistic character of . In particular populations, different traits were under selection. PR was high and similar in all populations in both years, while FRS was lower and varied among populations. Nectar was dominated by glucose, fructose, and included 28 AAs (Ala and Glu have the highest content). Sugars and AAs influenced mainly FRS. Among soil parameters, carbon and carbon:nitrogen ratio seems to be the most important in shaping flower structure and nectar chemistry.
植物-传粉者相互作用显著影响生殖成功（RS），并推动传粉综合征的进化。在 RS 的背景下，主要涉及花形态的作用。尽管花蜜性质在传粉效率中具有重要意义，但花蜜性质的重要性研究较少。因此，本研究旨在测试广布兰花的花形态和花蜜化学的选择。在 2019-2020 年，我们测量了三种花展示和六种花特征、花粉去除（PR）、雌性生殖成功（FRS），并确定了土壤性质。使用 HPLC 法分析了糖和氨基酸（AAs）。使用多种统计方法（箱线图、三元图、单向方差分析、克鲁斯卡尔-沃利斯检验和 PCA）分析数据。花结构和花蜜化学的变化及其与 RS 的弱相关性证实了 的广布性。在特定的种群中，不同的特征受到选择。PR 在两年的所有种群中都很高且相似，而 FRS 较低且在种群之间存在差异。花蜜以葡萄糖、果糖为主，并包含 28 种氨基酸（Ala 和 Glu 含量最高）。糖和氨基酸主要影响 FRS。在土壤参数中，碳和碳：氮比似乎在塑造花结构和花蜜化学方面最为重要。
