Kaur Kawaljit, Vaziri Shahram, Romero-Reyes Marcela, Paranjpe Avina, Jewett Anahid
Division of Oral Biology and Oral Medicine, School of Dentistry and Medicine, Los Angeles, CA 90095, USA.
Department of Neural and Pain Sciences, University of Maryland, Baltimore, MD 21201, USA.
J Clin Med. 2021 Feb 20;10(4):875. doi: 10.3390/jcm10040875.
Survival and function of immune subsets in the oral blood, peripheral blood and gingival tissues of patients with periodontal disease and healthy controls were assessed. NK and CD8 + T cells within the oral blood mononuclear cells (OBMCs) expressed significantly higher levels of CD69 in patients with periodontal disease compared to those from healthy controls. Similarly, TNF-α release was higher from oral blood of patients with periodontal disease when compared to healthy controls. Increased activation induced cell death of peripheral blood mononuclear cells (PBMCs) but not OBMCs from patients with periodontal disease was observed when compared to those from healthy individuals. Unlike those from healthy individuals, OBMC-derived supernatants from periodontitis patients exhibited decreased ability to induce secretion of IFN-γ by allogeneic healthy PBMCs treated with IL-2, while they triggered significant levels of TNF-α, IL-1β and IL-6 by untreated PBMCs. Interaction of PBMCs, or NK cells with intact or NFκB knock down oral epithelial cells in the presence of a periodontal pathogen, significantly induced a number of pro-inflammatory cytokines including IFN-γ. These studies indicated that the relative numbers of immune subsets obtained from peripheral blood may not represent the composition of the immune cells in the oral environment, and that orally-derived immune effectors may differ in survival and function from those of peripheral blood.
评估了牙周病患者和健康对照者口腔血液、外周血及牙龈组织中免疫亚群的存活情况和功能。与健康对照者相比，牙周病患者口腔血单核细胞（OBMC）中的自然杀伤细胞（NK）和CD8 + T细胞表达的CD69水平显著更高。同样，与健康对照者相比，牙周病患者口腔血液中的肿瘤坏死因子-α（TNF-α）释放量更高。与健康个体相比，观察到牙周病患者外周血单核细胞（PBMC）而非OBMC的活化诱导细胞死亡增加。与健康个体不同，牙周炎患者的OBMC来源的上清液诱导用白细胞介素-2（IL-2）处理的同种异体健康PBMC分泌干扰素-γ（IFN-γ）的能力下降，而它们能通过未处理的PBMC引发显著水平的TNF-α、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）。在牙周病原体存在的情况下，PBMC或NK细胞与完整或核因子κB（NFκB）敲低的口腔上皮细胞相互作用，显著诱导了包括IFN-γ在内的多种促炎细胞因子。这些研究表明，从外周血获得的免疫亚群的相对数量可能无法代表口腔环境中免疫细胞的组成，并且口腔来源的免疫效应器在存活和功能上可能与外周血的不同。
