Post-Graduation Program in Biotechnology and Biodiversity, Federal University of Goiás, Goiânia, Brazil; Biological Research Laboratory, Goiano Federal Institute - Urutaí Campus, Urutaí, Brazil.
Post-Graduation Program in Biotechnology and Biodiversity, Federal University of Goiás, Goiânia, Brazil.
Aquat Toxicol. 2021 Apr;233:105795. doi: 10.1016/j.aquatox.2021.105795. Epub 2021 Feb 27.
Although carbon nanotubes' (CNTs) toxicity in different experimental systems (in vivo and in vitro) is known, little is known about the toxic effects of carbon nanofibers (CNFs) on aquatic vertebrates. We herein investigated the potential impact of CNFs (1 and 10 mg/L) by using Physalaemus cuvieri tadpoles as experimental model. CNFs were able to induce nutritional deficit in animals after 48-h exposure to them, and this finding was inferred by reductions observed in body concentrations of total soluble carbohydrates, total proteins, and triglycerides. The increased production of hydrogen peroxide, reactive oxygen species and thiobarbituric acid reactive substances in tadpoles exposed to CNFs has suggested REDOX homeostasis change into oxidative stress. This process was correlated to the largest number of apoptotic and necrotic cells in the blood of these animals. On the other hand, the increased superoxide dismutase and catalase activity has suggested that the antioxidant system of animals exposed to CNFs was not enough to maintain REDOX balance. In addition, CNFs induced increase in acetylcholinesterase and butyrylcholinesterase activity, as well as changes in the number of neuromasts evaluated on body surface (which is indicative of the neurotoxic effect of nanomaterials on the assessed model system). To the best of our knowledge, this is the first report on the impact of CNFs on amphibians; therefore, it broadened our understanding about ecotoxicological risks associated with their dispersion in freshwater ecosystems and possible contribution to the decline in the populations of anurofauna species.
尽管已知碳纳米管（CNTs）在不同实验系统（体内和体外）中的毒性，但对于碳纳米纤维（CNFs）对水生脊椎动物的毒性影响知之甚少。我们在此使用 Physalaemus cuvieri 蝌蚪作为实验模型，研究了 CNFs 的潜在影响（1 和 10 mg/L）。CNFs 在暴露于它们 48 小时后能够诱导动物营养不足，这一发现是通过观察到动物体内总可溶性碳水化合物、总蛋白质和甘油三酯的浓度降低而推断出来的。暴露于 CNFs 的蝌蚪中过氧化氢、活性氧和硫代巴比妥酸反应物质的产生增加表明氧化还原平衡向氧化应激转变。这一过程与这些动物血液中凋亡和坏死细胞数量的增加有关。另一方面，超氧化物歧化酶和过氧化氢酶活性的增加表明，暴露于 CNFs 的动物的抗氧化系统不足以维持氧化还原平衡。此外，CNFs 还诱导乙酰胆碱酯酶和丁酰胆碱酯酶活性的增加，以及身体表面评估的神经丘数量的变化（这表明纳米材料对评估模型系统的神经毒性作用）。据我们所知，这是关于 CNFs 对两栖动物影响的首次报道，因此，它拓宽了我们对与它们在淡水生态系统中分散相关的生态毒理学风险以及对无尾目动物种群减少的可能贡献的认识。
