Cohn V H, Moore G P
Division of Biological Sciences, University of Michigan, Ann Arbor.
Mol Biol Evol. 1988 Mar;5(2):154-66. doi: 10.1093/oxfordjournals.molbev.a040487.
The alcohol dehydrogenase (Adh) gene was isolated from Drosophila simulans and D. mauritiana, and the DNA sequence of a 4.6-kb region, containing the structural gene and flanking sequence, was determined for each. These sequences were compared with the Adh region of D. melanogaster to characterize changes that occur in the Drosophila genome during evolution and to identify conserved sequences of functional importance. Drosophila simulans and D. mauritiana Adh are organized in a manner similar to that of D. melanogaster Adh, including the presence of two promoters for the single Adh gene. This study identified conserved flanking elements that, in conjunction with other studies, suggest regions that may be involved in the control of Adh expression. Inter- and intraspecies comparisons revealed differences in the kinds of sequence changes that have accumulated. Sequence divergence in and around the Adh gene was used to assess inter- and intraspecies evolutionary relationships. Finally, there appears to be an unrelated structural gene located directly 3' of the Adh transcribed region.
从拟果蝇和毛里求斯果蝇中分离出乙醇脱氢酶（Adh）基因，并测定了每个基因中包含结构基因和侧翼序列的4.6kb区域的DNA序列。将这些序列与黑腹果蝇的Adh区域进行比较，以表征果蝇基因组在进化过程中发生的变化，并识别具有功能重要性的保守序列。拟果蝇和毛里求斯果蝇的Adh基因的组织方式与黑腹果蝇的Adh基因相似，包括单个Adh基因存在两个启动子。这项研究确定了保守的侧翼元件，结合其他研究，表明这些区域可能参与Adh表达的调控。种间和种内比较揭示了积累的序列变化类型的差异。Adh基因及其周围的序列差异被用于评估种间和种内的进化关系。最后，在Adh转录区域的3'端似乎直接存在一个不相关的结构基因。
