2 型糖尿病患者脂肪组织 CD14 细胞基因表达的变化及其与环境因素的关系。
Changes in gene expression of adipose tissue CD14 cells in patients with Type 2 diabetes mellitus and their relationship with environmental factors.
机构信息
School of Public Health, Shaanxi University of Chinese Medicine, Xianyang Shaanxi 712046.
School of Public Health, Lanzhou University, Lanzhou 730000, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2021 Jan 28;46(1):1-10. doi: 10.11817/j.issn.1672-7347.2021.190558.
OBJECTIVES
To study the gene expression of adipose tissue CD14 cells in patients with Type 2 diabetes mellitus (T2DM) based on chip data, screen differentially expressed genes, and analyze their relationship with the environmental factors.
METHODS
The data of GSE54350 were obtained from the public database of gene expression profiling. The data were pre-processed by Network Analyst, String 11.0, Cytoscape 3.7.1, and other analytical software. The differentially expressed genes were analyzed by gene ontology biological function and kyoto encycopedia of genes and genomes (KEGG) signaling pathway to establish differential gene protein interaction network, transcription factor-gene regulatory network, microRNA-gene regulatory network, environmental factors-gene regulatory network, and other interaction systems.
RESULTS
The gene expression pattern of CD14 cells in adipose tissue of obese T2DM patients was significantly different from that of obese non-T2DM patients. There were 19 differentially expressed genes with up-regulation. The differentially expressed genes were mainly involved in ARP2/3 complex regulation of actin cytoskeleton, positively associated with biological processes such as protein complex assembly, and involved in the phagocytic Fcγ receptor signaling pathways and receptor family signaling pathways. The protein-protein interaction networks showed that TNF was the core protein node. The microRNA-gene regulatory network showed that hsa-mir-124-3p interacted with differentially expressed genes; TNF, KYNU, RCAN1 and other related genes all interacted with environmental factors.
CONCLUSIONS
The gene expression of adipose tissue CD14 cells are significantly changed in obese T2DM patients. TNF may play an important role in the process of obesity affecting the immune status of T2DM patients. Multiple microRNAs, transcription factors, and environmental factors also play a role in the above process. This study provides new material and new ideas for further exploration of the impact of obesity on T2DM patients.
目的
基于芯片数据研究 2 型糖尿病（T2DM）患者脂肪组织 CD14 细胞的基因表达，筛选差异表达基因，并分析其与环境因素的关系。
方法
从基因表达谱公共数据库中获取 GSE54350 数据，应用 Network Analyst、String 11.0、Cytoscape 3.7.1 等分析软件对数据进行预处理，采用基因本体生物功能和京都基因与基因组百科全书（KEGG）信号通路分析差异表达基因，构建差异基因蛋白互作网络、转录因子-基因调控网络、miRNA-基因调控网络、环境因素-基因调控网络等交互系统。
结果
肥胖 T2DM 患者脂肪组织 CD14 细胞的基因表达模式与肥胖非 T2DM 患者存在显著差异，有 19 个差异表达基因呈上调趋势。差异表达基因主要参与 ARP2/3 复合物调节肌动蛋白细胞骨架、与蛋白复合物组装等生物学过程呈正相关，涉及吞噬 Fcγ 受体信号通路和受体家族信号通路。蛋白-蛋白互作网络显示 TNF 是核心蛋白节点。miRNA-基因调控网络显示 hsa-mir-124-3p 与差异表达基因相互作用；TNF、KYNU、RCAN1 等相关基因均与环境因素相互作用。
结论
肥胖 T2DM 患者脂肪组织 CD14 细胞的基因表达发生明显变化，TNF 可能在肥胖影响 T2DM 患者免疫状态的过程中发挥重要作用，多种 miRNA、转录因子和环境因素也在上述过程中发挥作用。本研究为进一步探讨肥胖对 T2DM 患者的影响提供了新的材料和思路。
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