通过转染靶向氨基糖苷-羧基烷基聚乙烯亚胺纳米复合物的小干扰RNA沉默基因可降低MCF-7乳腺癌细胞的迁移能力。
Silencing Gene by Transfecting Small Interfering RNA with Targeted Aminoglycoside-Carboxyalkyl Polyethylenimine Nano-Polyplexes Reduced Migration of MCF-7 Breast Cancer Cells.
作者信息
Noruzi Somaye, Vatanchian Mehran, Azimian Amir, Niroomand Arash, Salarinia Reza, Oroojalian Fatemeh
机构信息
Department of Advanced Sciences and Technologies, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran.
Student Research Committee, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran.
出版信息
Avicenna J Med Biotechnol. 2021 Jan-Mar;13(1):2-8. doi: 10.18502/ajmb.v13i1.4580.
BACKGROUND
The application of non-viral systems for delivering genes to cells is becoming a very interesting issue, especially in the treatment of neoplasms such as Breast Cancer (BC). Polymer-based non-viral systems are safe and feasible gene carriers to be used in targeted cancer therapy. gene encodes a transcription factor and is overexpressed in some cancers.
METHODS
In this study, carboxyalkylated-PEI25 (25 ) was used to deliver plasmids expressing -siRNA into MCF-7 cells. DLS and AFM were applied to determine the size of nanoparticles. The MTT method was used to assess cytotoxicity, and the efficiency of transfection was confirmed both qualitatively and quantitatively. Finally, the effect of silencing was investigated on the migration of MCF7 cells using the scratch test.
RESULTS
The results showed that transferring the -siRNA using PEI25G10C50 reduced the expression of the corresponding transcription factor by 14 folds which attenuated the migration of MCF-7 cells by 58%.
CONCLUSION
In conclusion, PEI25G10C50 can serve as an effective gene delivery system for treating BC by targeting .
背景
将非病毒系统应用于向细胞递送基因正成为一个非常有趣的问题，尤其是在治疗诸如乳腺癌（BC）等肿瘤方面。基于聚合物的非病毒系统是用于靶向癌症治疗的安全且可行的基因载体。某基因编码一种转录因子，且在某些癌症中过表达。
方法
在本研究中，使用羧烷基化-PEI25（25 ）将表达 -siRNA的质粒递送至MCF-7细胞。应用动态光散射（DLS）和原子力显微镜（AFM）来测定纳米颗粒的大小。采用MTT法评估细胞毒性，并从定性和定量两方面确认转染效率。最后，使用划痕试验研究沉默 对MCF7细胞迁移的影响。
结果
结果表明，使用PEI25G10C50转染 -siRNA可使相应转录因子的表达降低14倍，这使MCF-7细胞的迁移减弱了58%。
结论
总之，PEI25G10C50可作为一种有效的基因递送系统，通过靶向 来治疗乳腺癌。
Zotero 插件