一种在小鼠中建立人肺腺癌细胞系异种移植模型的方法。

A Method for the Establishment of Human Lung Adenocarcinoma Patient-Derived Xenografts in Mice.

机构信息

Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, VIC, Australia.

Department of Molecular and Translational Sciences, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, VIC, Australia.

出版信息

Methods Mol Biol. 2021;2279:165-173. doi: 10.1007/978-1-0716-1278-1_13.

DOI:10.1007/978-1-0716-1278-1_13

PMID:33683693

Abstract

Patient-derived xenografts (PDXs) are created by implanting human tumor tissue or cells into immunodeficent mice, and enable the study of tumor biology, biomarkers and response to therapy in vivo. This chapter describes a method for lung adenocarcinoma (LAC) PDX generation using subcutaneous implantation of tumor tissue and cell suspensions and incorporating the humanization of PDX models by reconstitution with human immune cells.

摘要

患者来源的异种移植物（PDXs）是通过将人类肿瘤组织或细胞植入免疫缺陷小鼠中创建的，使人们能够在体内研究肿瘤生物学、生物标志物和对治疗的反应。本章描述了一种使用皮下植入肿瘤组织和细胞悬液生成肺腺癌（LAC）PDX 的方法，并通过用人类免疫细胞重建来实现 PDX 模型的人源化。