早产儿脐血来源的外周血单个核细胞与内皮祖细胞的共培养。

Co-Culture of Peripheral Blood Mononuclear Cells and Endothelial Colony Forming Cells from Cord Blood of Preterm Born Babies.

机构信息

Section for Neonatology and Pediatric Intensive Care, Department for Operative and Nonoperative Pediatric and Adolescent Medicine, University Hospital Halle (Saale), Medical Faculty of Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.

Department for Operative and Nonoperative Pediatric and Adolescent Medicine, University Clinic and Outpatient Clinic for Pediatrics I, University Hospital Halle (Saale), Medical Faculty of Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.

出版信息

Methods Mol Biol. 2021;2269:107-124. doi: 10.1007/978-1-0716-1225-5_8.

DOI:10.1007/978-1-0716-1225-5_8

PMID:33687675

Abstract

Umbilical cord blood of neonates is a precious source for many fields of research because of distinct unique features combined with easy accessibility at the time of birth. The number of applications are vast with an emphasis in the field of stem cell therapy and regenerative medicine since cord blood contains relatively large numbers of pluripotent cells. This chapter provides a protocol for developing an autologous co-culture of endothelial-like cells and peripheral blood mononuclear cells from umbilical cord blood of premature born babies and describes an experimental setting to investigate inflammatory processes that are a cornerstone of pathophysiology in the developing organs of preterm born babies.

摘要

新生儿脐带血因其独特的特性，且易于在出生时获取，成为许多研究领域的宝贵资源。脐带血中含有相对较多的多能细胞，因此应用广泛，特别是在干细胞治疗和再生医学领域。本章提供了一种从早产儿脐带血中分离内皮样细胞和外周血单个核细胞并进行自体共培养的方案，并描述了一种实验设置，用于研究炎症过程，这是早产儿发育中器官病理生理学的基石。