Plant Pathology Department, Faculty of Agriculture, Assiut University, Assiut, 71526, Egypt.
Mol Biol Rep. 2021 Mar;48(3):3001-3006. doi: 10.1007/s11033-021-06254-8. Epub 2021 Mar 9.
Tomato plants displaying early blight symptoms were collected from different localities in the provinces of Assiut and Sohag, Egypt. The causal pathogens were isolated on potato dextrose agar plates. Pathogenicity tests with 48 isolates were carried out under greenhouse conditions on tomato cultivar (CV 844). All tested isolates caused symptoms of early blight disease with different degrees. The highest disease severity on tomato plants was found after inoculation with isolate No. 6 followed by isolates No. 20 and No. 31. The most pathogenic isolates were identified by sequence analysis using ITS1 and ITS4 primers. The analysis of the amplified sequences from fungal isolates No. 6, 20 and 31 displayed 99-100% nucleotide identity with Alternaria solani, Curvularia lunata and A. alternata, respectively. To our knowledge, this is the first report of Curvularia lunata as one of the causal pathogens of early blight disease of tomato plants in Egypt.
从埃及阿西尤特省和索哈杰省的不同地区采集到表现早疫病症状的番茄植株。在马铃薯葡萄糖琼脂平板上分离出病原菌。在温室条件下，对番茄品种（CV 844）的 48 个分离物进行了致病性试验。所有测试的分离物都引起了不同程度的早疫病症状。用 6 号分离物接种后，番茄植株的严重程度最高，其次是 20 号和 31 号分离物。用 ITS1 和 ITS4 引物进行序列分析，鉴定出最具致病性的分离物。从真菌分离物 6、20 和 31 扩增的序列分析显示，核苷酸同源性分别为 99-100%与茄丝核菌、旋孢腔菌和交链孢菌。据我们所知，这是旋孢腔菌作为埃及番茄早疫病病原菌之一的首次报道。
