利用端粒酶催化亚单位实现人角质形成细胞永生化。

Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.

Pirogov Russian National Research Medical University, Moscow, Russia.

Dokl Biochem Biophys. 2021 May;496(1):5-9. doi: 10.1134/S1607672921010014. Epub 2021 Mar 10.

A new stable line of human keratinocytes was obtained. The cells have altered morphology, both abnormal chromosomal composition and expression of keratinocyte markers, do not show contact inhibition, could be cultured in various media and have limited stratification ability in vitro. Upon transplantation into nude mice the cells have tumorigenic properties.

获得了一条新的人角质形成细胞稳定系。这些细胞的形态发生了改变，既有异常的染色体组成，又有角蛋白标记物的表达异常，不表现接触抑制，能在多种培养基中培养，体外有限的分层能力。移植到裸鼠后，这些细胞具有致瘤性。