暴露于阿霉素的培养新生大鼠心脏细胞中的乳酸脱氢酶活性

Lactate dehydrogenase activity in cultured neonatal rat heart cells exposed to doxorubicin.

作者信息

Howard P H, Payne S, Wong L, Gonzalez B, Lewis W

机构信息

Department of Pathology, UCLA School of Medicine Center for the Health Sciences 90024.

出版信息

Exp Mol Pathol. 1988 Jun;48(3):311-6. doi: 10.1016/0014-4800(88)90067-6.

DOI:10.1016/0014-4800(88)90067-6

PMID:3371455

Abstract

Doxorubicin (Adriamycin, ADR) is an anthracycline antineoplastic with the serious side effect of dose-related cardiomyopathy. A model of ADR cardiotoxicity was created to examine some subcellular toxic effects of ADR with cultured cardiac myocytes (CMCs) exposed to 1 x 10(-7) to 1 x 10(-5) M ADR for 24 to 48 hr. Lactate dehydrogenase (LDH) activity was monitored in the CMC medium to monitor CMC damage as a function of ADR concentration. A four- to eightfold elevation of LDH activity in medium of CMCs exposed to 1 x 10(-6) to 1 x 10(-5) M ADR was found. No change in LDH activity was detected in medium of CMCs exposed to 1 x 10(-7) M ADR or in control CMCs after 24 or 48 h ADR exposure. Data suggest a dose-dependent effect of ADR on LDH activity in CMC medium. Serial monitoring of LDH in media of ADR-exposed CMCs may correlate with other evidence of ADR cardiotoxicity in vitro.

摘要

多柔比星（阿霉素，ADR）是一种蒽环类抗肿瘤药物，具有与剂量相关的心肌病这一严重副作用。构建了ADR心脏毒性模型，以研究ADR对培养的心肌细胞（CMC）的一些亚细胞毒性作用，将CMC暴露于1×10⁻⁷至1×10⁻⁵ M的ADR中24至48小时。监测CMC培养基中的乳酸脱氢酶（LDH）活性，以监测作为ADR浓度函数的CMC损伤情况。发现暴露于1×10⁻⁶至1×10⁻⁵ M ADR的CMC培养基中LDH活性升高了4至8倍。在暴露于1×10⁻⁷ M ADR的CMC培养基中或在ADR暴露24或48小时后的对照CMC中，未检测到LDH活性变化。数据表明ADR对CMC培养基中LDH活性具有剂量依赖性效应。对暴露于ADR的CMC培养基中的LDH进行连续监测可能与体外ADR心脏毒性的其他证据相关。