通过原位杂交研究雌激素受体在大鼠垂体中的分布。

Distribution of estrogen receptors in the rat pituitary as studied by in situ hybridization.

作者信息

Pelletier G, Liao N, Follea N, Govindan M V

机构信息

MRC Group in Molecular Endocrinology, Le Centre Hospitalier de l'Université Laval, Quebec, Que, Canada.

出版信息

Mol Cell Endocrinol. 1988 Mar;56(1-2):29-33. doi: 10.1016/0303-7207(88)90005-6.

DOI:10.1016/0303-7207(88)90005-6

PMID:3371545

Abstract

The localization of estrogen receptors in the rat pituitary gland was performed by in situ hybridization. The probe used was a synthetic oligonucleotide probe labelled with 35S complementary to the mRNA coding for a fragment (1-24) of estrogen receptor. Strong labelling was observed in cells of the anterior and intermediate lobes whereas the posterior lobe remains unlabelled. These results suggest that not only the anterior lobe but also the intermediate lobe is a target for estrogens.

摘要

通过原位杂交法对大鼠垂体中雌激素受体进行定位。所用探针是一种用35S标记的合成寡核苷酸探针，其与编码雌激素受体片段（1 - 24）的mRNA互补。在前叶和中叶细胞中观察到强标记，而后叶未标记。这些结果表明，不仅前叶，中叶也是雌激素的作用靶点。