Department of Food Science, Fu Jen Catholic University, New Taipei City 242, Taiwan.
Research Center for Chinese Herbal Medicine and Research Center for Food and Cosmetic Safety, Chang Gung University of Science and Technology, Taoyuan City 333, Taiwan.
Food Chem. 2021 Aug 15;353:129474. doi: 10.1016/j.foodchem.2021.129474. Epub 2021 Mar 7.
This study aims to determine toxic compounds polycyclic aromatic hydrocarbons (PAHs) and cholesterol oxidation products (COPs) in thin slices of dried pork as affected by different flavorings and roasting temperature treatments through employing a QuEChERS method coupled with gas chromatograph-tandem mass spectrometer (GC-MS/MS) and gas chromatograph-mass spectrometer (GC-MS), respectively. By employing this method, high accuracy and precision was attained for freeze-dried pork hind leg sample. Following addition of 8 different flavorings with roasting temperature at 120, 160, and 200 °C, the levels of total COPs and PAHs in thin slices of dried pork followed a temperature-dependent increase during roasting, which was further confirmed by principle component analysis. High level of soy sauce or sugar inhibited COP formation, while the low-level minimized PAH formation in thin slices of dried pork during roasting. Sugar was more effective in inhibiting COP formation while soy sauce was more efficient in reducing PAH formation.
本研究旨在通过 QuEChERS 方法结合气相色谱-串联质谱(GC-MS/MS)和气相色谱-质谱(GC-MS),分别研究不同调味剂和烘烤温度处理对干猪肉薄片中多环芳烃(PAHs)和胆固醇氧化产物(COPs)等有毒化合物的影响。采用该方法对冷冻干燥猪后腿样品进行分析,结果具有较高的准确度和精密度。在 120、160 和 200°C 的烘烤温度下加入 8 种不同的调味料后,烘烤过程中干猪肉薄片中总 COPs 和 PAHs 的水平随温度升高而升高,这一点通过主成分分析得到了进一步证实。酱油或糖的高浓度水平会抑制 COP 的形成,而低浓度水平会减少烘烤过程中干猪肉薄片中 PAH 的形成。糖在抑制 COP 形成方面更有效,而酱油在减少 PAH 形成方面更有效。