Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; Key Laboratory of Aquatic Animal Diseases and Immune Technology of Heilongjiang Province, Harbin 150070, China.
Centre for Genetic Improvement of Livestock, Department of Animal Biosciences, University of Guelph, Guelph N1G2W1, Canada.
Comp Biochem Physiol Part D Genomics Proteomics. 2021 Jun;38:100824. doi: 10.1016/j.cbd.2021.100824. Epub 2021 Mar 15.
The endangered cold-water fish species taimen (Hucho taimen) suffer acute temperature changes in culture and wild conditions. Understanding the effects of acute temperature changes on physiological processes of this species is essential for aquaculture practices and conservation. Liver transcriptomic profiles of the taimen (n = 24) exposed to acute temperature decrease (from 20 °C to 10 °C) and acute temperature increase (from 10 °C to 20 °C) was evaluated using high-throughput RNA-Sequencing. Samples were collected at day 0, 1, 7 and 35 in both treatments. Compared to day 0, the total numbers of differentially expressed genes (DEGs) in the taimen after acute temperature decrease were 173, 226 and 42 at day 1, 7 and 35, respectively, and the total numbers of DEGs following acute temperature increase were 260, 253 and 282 at day 1, 7 and 35, respectively. Particularly, 14 key regulatory genes were commonly found between both acute temperature treatments. Functional analysis based on the commonly identified DEGs revealed important metabolic pathways related to metabolism and immune function, suggesting a specific response mechanism of taimen against cold and heat shock. The results may assist in developing management strategies for stress mediation caused by acute temperature changes in the taimen and other cold water fish.
濒危冷水鱼类哲罗鱼（Hucho taimen）在养殖和野生条件下会经历急性温度变化。了解这种物种在急性温度变化下的生理过程的影响对于水产养殖实践和保护至关重要。本研究使用高通量 RNA 测序评估了哲罗鱼（n = 24）暴露于急性温度下降（从 20°C 到 10°C）和急性温度上升（从 10°C 到 20°C）时的肝脏转录组谱。在两种处理中，分别在第 0、1、7 和 35 天采集样本。与第 0 天相比，急性温度下降后哲罗鱼的差异表达基因（DEGs）总数在第 1、7 和 35 天分别为 173、226 和 42，急性温度上升后哲罗鱼的差异表达基因（DEGs）总数分别为 260、253 和 282。特别是，两种急性温度处理之间共同发现了 14 个关键调控基因。基于共同鉴定的 DEGs 的功能分析揭示了与代谢和免疫功能相关的重要代谢途径，表明哲罗鱼对冷、热休克有特定的反应机制。这些结果可能有助于制定管理策略，以应对哲罗鱼和其他冷水鱼类因急性温度变化引起的应激。
