Arginase/nitric oxide modifications using live non-pathogenic as an effective delivery system inside the mammalian macrophages.

Recombinant live delivery system based on chemokine IFN-γ-inducible protein-10 kDa (CXCL 10 or IP-10), as a suitable immunotherapy tool, have been used for the treatment of infections. This chemokine can defeat spp. infection via producing nitric oxide (NO) for parasite killing. This study was performed to investigate the effects of IP-10 on the infected human macrophages by expressing IP-10. We also quantified the arginase activity and NO production in the co-cultured human macrophages with expressing IP-10 as compared with wild . The results elucidate that in the infected cells with expression of IP-10 the arginase activity decreased, and inversely, NO production intensely increased. Altogether, expressing IP-10 shows a favorable therapeutic tool to improve the treatment of infection. This work suggests that expressing IP-10 cause specific effects on the metabolic pathways of the macrophage host, which might enable the host cells in killing of parasites and decreasing the survival of them against infection.