Frey Jasmin, Kaßner Sophie, Schink Bernhard
Department of Biology, University of Konstanz, 78457, Konstanz, Germany.
Curr Microbiol. 2021 May;78(5):1763-1770. doi: 10.1007/s00284-021-02441-9. Epub 2021 Mar 22.
Degradation of acetone and higher ketones has been described in detail for aerobic and nitrate-reducing bacteria. Among sulfate-reducing bacteria, degradation of acetone and other ketones is still an uncommon ability and has not been understood completely yet. In the present work, we show that Desulfotomaculum arcticum and Desulfotomaculum geothermicum are able to degrade acetone and butanone. Total proteomics of cell-free extracts of both organisms indicated an involvement of a thiamine diphosphate-dependent enzyme, a B-dependent mutase, and a specific dehydrogenase during acetone degradation. Similar enzymes were recently described to be involved in acetone degradation by Desulfococcus biacutus. As there are so far only two described sulfate reducers able to degrade acetone, D. arcticum and D. geothermicum represent two further species with this capacity. All these bacteria appear to degrade acetone via the same set of enzymes and therefore via the same pathway.
关于需氧菌和硝酸盐还原菌对丙酮及高级酮类的降解已有详细描述。在硫酸盐还原菌中，丙酮和其他酮类的降解仍是一种不常见的能力，尚未被完全理解。在本研究中，我们发现北极脱硫肠状菌和地热脱硫肠状菌能够降解丙酮和丁酮。对这两种微生物无细胞提取物的全蛋白质组学分析表明，在丙酮降解过程中，一种硫胺二磷酸依赖性酶、一种B依赖性变位酶和一种特定的脱氢酶参与其中。最近有报道称，类似的酶参与了双尖脱硫球菌对丙酮的降解。由于迄今为止仅发现两种能够降解丙酮的硫酸盐还原菌，北极脱硫肠状菌和地热脱硫肠状菌是另外两种具有此能力的菌种。所有这些细菌似乎都通过同一组酶，因此通过相同的途径降解丙酮。
