Department of Zoology, Federal University of Parana, 82590-300 Curitiba, Brazil.
Laboratory of Entomology, Embrapa Wheat Research Center, 99050-970 Passo Fundo, Brazil.
J Insect Physiol. 2021 May-Jun;131:104228. doi: 10.1016/j.jinsphys.2021.104228. Epub 2021 Mar 19.
Laboratory studies were conducted with the Neotropical brown stink bug, Euschistus heros (F.), to evaluate nymphal and adult biology on immature pods of soybean, Glycine max (L.) Merrill (Fabaceae), bearing the block technology (resistant to stink bug damage - cvs. BRS 391, BRS 543 RR and BRS 1003 IPRO) compared to a susceptible cultivar (BRS 5601 RR). Results indicated that nymphs' developmental time and survivorship were similar on all cultivars tested. The same was observed for adult survivorship and reproductive performance. However, data from electropenetrography (EPG) demonstrated that adults of E. heros spent significantly less time in feeding activities on resistant plants compared to the susceptible one. Large differences were observed in feeding activities on seeds; on resistant plants, the insects dedicated a shorter period of time to feed on seed endosperm than on BRS 5601. In addition, when bugs fed on seeds of block cultivars, the majority of probes were composed of only laceration/maceration activities (Eh3a waveform) without ingestion events of the cell contents (Eh3b waveform). In contrast, on the susceptible cultivar, Eh3a waveform events were repeated much more frequently (3-5X) with more probes also containing ingestion of seed contents. These results suggest that the soybean cultivars bearing the block technology presented a lower preference (antixenosis) by the bugs with fewer feeding activities, primarily in the seed endosperm, compared to the susceptible one tested.
实验室研究表明，与易受臭虫伤害的品种（BRS 5601 RR）相比，在含有阻遏技术（抗臭虫损伤- cvs. BRS 391、BRS 543 RR 和 BRS 1003 IPRO）的未成熟大豆荚（Glycine max（L.）Merrill（豆科））上，新热带棕色臭虫 Euschistus heros（F.）的若虫和成虫生物学特性相似。结果表明，在所有测试的品种上，若虫的发育时间和存活率相似。成虫的存活率和生殖性能也是如此。然而，电穿孔图（EPG）的数据表明，与易感品种相比，E. heros 成虫在抗性植物上的取食活动时间明显减少。在种子上的取食活动有很大差异；在抗性植物上，昆虫在种子胚乳上的取食时间比 BRS 5601 更短。此外，当臭虫以阻遏品种的种子为食时，大多数探针仅由切割/粉碎活动（Eh3a 波型）组成，而没有细胞内容物的摄取事件（Eh3b 波型）。相比之下，在易感品种上，Eh3a 波型事件更频繁地重复（3-5 倍），并且更多的探针也包含对种子内容物的摄取。这些结果表明，与测试的易感品种相比，含有阻遏技术的大豆品种对臭虫的偏好性较低（抗生性），取食活动较少，主要是在种子胚乳中。
