生长激素 1 基因与中国本土牦牛（Bos grunniens）生长性状关联的分子特征与分析。

Molecular characterization and analysis of the association of growth hormone 1 gene with growth traits in Chinese indigenous yak (Bos grunniens).

机构信息

College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province, 810016, People's Republic of China.

State Key Laboratory of Animal Genetics Breeding & Reproduction, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.

出版信息

Trop Anim Health Prod. 2021 Mar 22;53(2):221. doi: 10.1007/s11250-021-02671-w.

DOI:10.1007/s11250-021-02671-w

PMID:33754201

Abstract

This study aimed to investigate the effects of polymorphisms in growth hormone 1 (GH1) gene on the growth traits in Chinese indigenous yak. Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-PFLP) approach, one novel single-nucleotide polymorphism (SNP), termed as g.1721G>A, was identified in the exon 4 of GH1 gene in 423 individuals of yak population. Based on the chi-square (χ) test, the frequencies of g.1721G>A alleles agreed with Hardy-Weinberg equilibrium (HWE) (P < 0.05). A significant association was observed between this SNP and several growth traits (P < 0.01 or P < 0.05), in which the genotype GG exhibited the best values. The present study suggested that the identified SNP was a useful genetic marker for the improvement of growth traits in Chinese indigenous yak.

摘要

本研究旨在探讨生长激素 1 (GH1) 基因多态性对中国本土牦牛生长性状的影响。使用聚合酶链反应-限制性片段长度多态性 (PCR-PFLP) 方法，在 423 头牦牛群体中鉴定出 GH1 基因外显子 4 中的一个新的单核苷酸多态性 (SNP)，称为 g.1721G>A。基于卡方 (χ) 检验，g.1721G>A 等位基因的频率符合 Hardy-Weinberg 平衡 (HWE) (P < 0.05)。该 SNP 与多个生长性状之间存在显著关联 (P < 0.01 或 P < 0.05)，其中 GG 基因型表现出最佳值。本研究表明，鉴定出的 SNP 是改良中国本土牦牛生长性状的有用遗传标记。