Department of Thoracic Surgery, The Yuebei People's Hospital of Shaoguan, Shaoguan, Guangdong 512025, P.R. China.
Department of Nursing, Medical College of Shaoguan University, Shaoguan, Guangdong 512026, P.R. China.
Oncol Rep. 2021 May;45(5). doi: 10.3892/or.2021.8018. Epub 2021 Mar 24.
Accumulating studies have suggested that microRNAs (miRs) play a significant role in lung cancer development and progression, especially in non‑small cell lung cancer (NSCLC). The present study aimed to investigate the associations between miR‑454‑3p and NSCLC progression. qPCR assay was applied to examine the expression of miR‑454‑3p and transforming growth factor‑β2 (TGFB2) in tissues and cell lines. CCK‑8 and EdU assays were used to detect cell proliferation. Wound‑healing and Transwell assays were conducted to assess cell migration and invasion. Western blotting assay was performed to explore the protein levels of epithelial‑mesenchymal transition (EMT) markers. The interaction between miR‑454‑3p and TGFB2 was investigated with a luciferase reporter assay. miR‑454‑3p was downregulated in NSCLC tissues and NSCLC cell lines. miR‑454‑3p overexpression led to the suppression of proliferation, migration, and invasion in A549 and NCI‑H1650 cells. In addition, the overexpression of miR‑454‑3p in A549 and NCI‑H1650 cells significantly inhibited EMT. TGFB2 was revealed to be a direct target of miR‑454‑3p by using TargetScan database and luciferase reporter assay. TGFB2 was observed to be upregulated in NSCLC tissues and cell lines. Further mechanistic studies revealed that the inhibitory effects of miR‑454‑3p on NSCLC were reversed upon overexpression of TGFB2. These findings provided strong evidence that miR‑454‑3p suppressed NSCLC cell proliferation and metastasis by targeting TGFB2. The study suggests that targeting miR‑454‑3p could be a promising strategy for treating NSCLC.
越来越多的研究表明，微小 RNA（miRs）在肺癌的发生和发展中发挥着重要作用，尤其是在非小细胞肺癌（NSCLC）中。本研究旨在探讨 miR-454-3p 与 NSCLC 进展之间的关系。qPCR 检测用于检测组织和细胞系中 miR-454-3p 和转化生长因子-β2（TGFB2）的表达。CCK-8 和 EdU 检测用于检测细胞增殖。划痕愈合和 Transwell 检测用于评估细胞迁移和侵袭。Western blot 检测用于探索上皮间质转化（EMT）标志物的蛋白水平。通过荧光素酶报告基因检测研究 miR-454-3p 和 TGFB2 之间的相互作用。miR-454-3p 在 NSCLC 组织和 NSCLC 细胞系中下调。miR-454-3p 过表达导致 A549 和 NCI-H1650 细胞增殖、迁移和侵袭受到抑制。此外，miR-454-3p 在 A549 和 NCI-H1650 细胞中的过表达显著抑制 EMT。使用 TargetScan 数据库和荧光素酶报告基因检测证实 TGFB2 是 miR-454-3p 的直接靶标。TGFB2 在 NSCLC 组织和细胞系中上调。进一步的机制研究表明，miR-454-3p 对 NSCLC 的抑制作用在 TGFB2 过表达时被逆转。这些发现为 miR-454-3p 通过靶向 TGFB2 抑制 NSCLC 细胞增殖和转移提供了有力证据。研究表明，靶向 miR-454-3p 可能是治疗 NSCLC 的一种有前途的策略。
