Institute of Medical Science Research, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Department of Cardiac Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Mol Med Rep. 2021 May;23(5). doi: 10.3892/mmr.2021.12009. Epub 2021 Mar 24.
Nasopharyngeal carcinoma (NPC) is an epithelial carcinoma originating from the nasopharyngeal mucosal tissue and is highly prevalent in southeast Asia. Galectin‑3 (gal‑3) serves crucial roles in many cancers but its role in NPC remains to be elucidated. The aim of the present study was to investigate the role of gal‑3 in NPC. Immunohistochemistry and ELISA were used to determine the expression level of gal‑3 in patients with NPC or chronic rhinitis (CR). Gal‑3 short hairpin (sh)RNA was established to knockdown gal‑3 in 5‑8F and 6‑10B cells, allowing for the evaluation of the roles of gal‑3 in proliferation, migration and apoptosis in NPC cell lines. Immunohistochemistry staining of IL‑6 and IL‑8 was applied to access the inflammatory state of tumor tissues, and the correlation between the inflammatory state and gal‑3 was analyzed. The results demonstrated that gal‑3 was upregulated in patients with NPC compared with patients with CR. Knockdown of gal‑3 inhibited proliferation and migration in 5‑8F and 6‑10B cells, as well as promoted apoptosis in these cells. The expression levels of MMP‑9 and IL‑8 were also decreased in 5‑8F and 6‑10B cells after transfection with gal‑3 shRNA. A positive correlation was identified between the expression level of gal‑3 and the inflammatory state of NPC. The phosphorylation levels of ERK1/2 and Akt were downregulated after knockdown of gal‑3 in 5‑8F and 6‑10B cells. In conclusion, the expression level of gal‑3 was upregulated in patients with NPC and was positively correlated with the inflammatory state of NPC. The results suggested that gal‑3 promoted the proliferation and migration of 5‑8F and 6‑10B cells, while inhibiting the apoptosis of these cells. Moreover, activation of ERK1/2 and Akt may be the underlying mechanism of the effects of gal‑3 on NPC.
鼻咽癌（NPC）是一种上皮癌，起源于鼻咽黏膜组织，在东南亚地区高发。半乳糖凝集素-3（gal-3）在许多癌症中发挥着关键作用，但它在 NPC 中的作用仍有待阐明。本研究旨在探讨 gal-3 在 NPC 中的作用。免疫组织化学和 ELISA 用于测定 NPC 或慢性鼻炎（CR）患者中 gal-3 的表达水平。建立 gal-3 短发夹（sh）RNA 以敲低 5-8F 和 6-10B 细胞中的 gal-3，从而评估 gal-3 在 NPC 细胞系中的增殖、迁移和凋亡中的作用。应用 IL-6 和 IL-8 的免疫组织化学染色来评估肿瘤组织的炎症状态，并分析炎症状态与 gal-3 之间的相关性。结果表明，与 CR 患者相比，NPC 患者的 gal-3 表达上调。敲低 gal-3 抑制了 5-8F 和 6-10B 细胞的增殖和迁移，并促进了这些细胞的凋亡。转染 gal-3 shRNA 后，5-8F 和 6-10B 细胞中 MMP-9 和 IL-8 的表达水平也降低。gal-3 的表达水平与 NPC 的炎症状态呈正相关。敲低 gal-3 后，5-8F 和 6-10B 细胞中 ERK1/2 和 Akt 的磷酸化水平下调。综上所述，NPC 患者的 gal-3 表达上调，与 NPC 的炎症状态呈正相关。结果表明，gal-3 促进了 5-8F 和 6-10B 细胞的增殖和迁移，同时抑制了这些细胞的凋亡。此外，ERK1/2 和 Akt 的激活可能是 gal-3 对 NPC 影响的潜在机制。
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