Simonetti Amalia, Perna Annamaria, Grassi Giulia, Gambacorta Emilio
School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Viale dell'AteneoLucano, Potenza, 10-85100, Italy.
J Food Sci. 2021 Apr;86(4):1400-1409. doi: 10.1111/1750-3841.15661. Epub 2021 Mar 24.
Goat yogurt samples fortified with 20% (w/v) Rhus coriaria leaf powder were in vitro digested in order to evaluate the total phenolic content (TPC), antioxidant activity (AA), and bioaccessibility of phenolic compounds in the digestate. After digestion, TPC and AA values of the R. coriaria-fortified yogurts increased compared to the undigested yogurts (P < 0.001). In particular, TPC has increased about twice; whereas, AA values have increased about 10 and 6 times, for ABTS and FRAP assays, respectively. The bioaccessibility index was well above the 100% for all identified phenols; except for (-)-epicatechin (82.04%), rutin (51.51%), and gallic acid (5.42%). This different behavior highlighted that the bioaccessibility was modulated by both the yogurt-polyphenol complexes and phenol stability under digestion system. These findings can contribute to elucidate the influence of in vitro digestion on antioxidant capacity and polyphenols recovery infortified yogurts, and may help in the design of dairy products with better functional quality PRACTICAL APPLICATION: Rhus coriaria L. (Sumac) is a polyphenol-rich Mediterranean plant that may be used as functional ingredient to enrich fermented food such as yogurt. However, in fortified yogurts the evaluation of bioaccessibility, that is, the compounds released from the yogurt and stable in the digestive environment, thus able to exert their biological effects on the gastrointestinal system, is more important than the content of these compounds in the corresponding food. This study highlighted the phenolic content, antioxidant activity, and bioaccessibility of phenolic compounds in goat milk yogurt fortified with R. coriaria leaf powder after simulated gastro-pancreatic digestion.
对添加了20%(w/v)盐肤木叶粉的山羊酸奶样品进行体外消化,以评估消化产物中总酚含量(TPC)、抗氧化活性(AA)和酚类化合物的生物可及性。消化后,与未消化的酸奶相比,添加盐肤木的酸奶的TPC和AA值有所增加(P < 0.001)。特别是,TPC增加了约两倍;而对于ABTS和FRAP测定,AA值分别增加了约10倍和6倍。除(-)-表儿茶素(82.04%)、芦丁(51.51%)和没食子酸(5.42%)外,所有鉴定出的酚类化合物的生物可及性指数均远高于100%。这种不同的行为表明,生物可及性受酸奶-多酚复合物和消化系统中酚类稳定性的调节。这些发现有助于阐明体外消化对强化酸奶抗氧化能力和多酚回收的影响,并可能有助于设计具有更好功能品质的乳制品。实际应用:盐肤木是一种富含多酚的地中海植物,可作为功能性成分用于强化酸奶等发酵食品。然而,在强化酸奶中,评估生物可及性,即从酸奶中释放并在消化环境中稳定存在、从而能够对胃肠道系统发挥生物学作用的化合物,比相应食品中这些化合物的含量更为重要。本研究强调了模拟胃肠胰消化后添加盐肤木叶粉的山羊奶酸奶中酚类含量、抗氧化活性和酚类化合物的生物可及性。