dtoLABS, Via Pozzuoli, 13C/13D, 30038 Spinea (VE), Italy.
Department of Medical and Surgical Sciences, Unit of Legal Medicine, University of Bologna, Via Irnerio 49, 40126 Bologna, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 May 15;1172:122651. doi: 10.1016/j.jchromb.2021.122651. Epub 2021 Mar 10.
Biomonitoring of perfluoroalkyl substances (PFASs) in hair is conventionally achieved by SPE extraction and liquid chromatography-triple quadrupole analysis, with sensitivities in the range of ng/g. The aim of this study was to develop and validate a rapid method to detect 20 perfluoroalkyl substances (PFASs) in human hair from general populations by SPE purification and liquid-chromatography coupled to accurate mass measurement (LC-QTOF). The obtained sensitivities (LOQ), linearity and RSD accuracies were respectively in the range of 0.07-0.5 ng/g, 0.1 (or 0.2 or 0.5)-10 ng/g, 1-16%. To verify the applicability of the method, 11 hair samples from volunteers were tested. The detected PFAS were PFBA (range 0.24-14.6 ng/g), PFBS (0.496 ng/g), PFOA (range 0.08-0.178 ng/g) and PFOS (<LOQ-0.239 ng/g). The results were compared in terms of detection frequency and abundance with previously published studies. The method proved useful for the determination of the tested PFAS in the hair matrix.
头发中全氟烷基物质（PFASs）的生物监测通常通过固相萃取（SPE）提取和液相色谱-三重四极杆分析来实现，灵敏度范围在 ng/g 级。本研究旨在开发和验证一种通过 SPE 净化和液质联用（LC-QTOF）准确质量测量快速检测一般人群头发中 20 种全氟烷基物质（PFASs）的方法。所得的灵敏度（LOQ）、线性和 RSD 准确度分别在 0.07-0.5ng/g、0.1（或 0.2 或 0.5）-10ng/g 和 1-16%范围内。为了验证该方法的适用性，对 11 名志愿者的头发样本进行了测试。检测到的 PFAS 包括全氟丁酸（PFBA，范围为 0.24-14.6ng/g）、全氟丁基磺酸盐（PFBS，0.496ng/g）、PFOA（范围为 0.08-0.178ng/g）和 PFOS（<LOQ-0.239ng/g）。根据先前发表的研究，从检测频率和丰度方面对结果进行了比较。该方法证明可用于测定头发基质中的测试 PFAS。
