Wan Hongping, Ren Ke, Kaper Hans J, Sharma Prashant K
College of Veterinary Medicine, Sichuan Agricultural University, Department of Animal and Plant Quarantine, Chengdu 611130, China; University of Groningen and University Medical Center Groningen, Department of Biomedical Engineering, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands.
University of Groningen and University Medical Center Groningen, Department of Biomedical Engineering, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands.
J Colloid Interface Sci. 2021 Jul 15;594:435-445. doi: 10.1016/j.jcis.2021.03.052. Epub 2021 Mar 17.
Biomaterials that are used in biological systems, such as polycarbonate urethane (PCU) knee joint implants and contact lenses, generally lack lubrication. This limits their integration with the body and impedes their function. Here, we propose a nanostructured film based on hydrophilic polysaccharide hyaluronic acid conjugated with dopamine (HADN) and zwitterionic reduced glutathione (Glu), which forms a composite coating (HADN-Glu) to enhance the lubrication between cartilage and PCU. HADN was synthesized by carbodiimide chemistry between hyaluronic acid and dopamine and deposited on PCU surface under mild oxidative conditions. Then, zwitterionic peptide-reduced glutathione was bioconjugated to HADN, forming a lubrication film. Analysis based on X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM) and wettability indicated that HADN and Glu had grafted successfully onto the PCU surface. Measurements of the coefficient of friction (COF), friction energy dissipation and cartilage roughness indicated that cartilage was effectively protected by the high lubrication of HADN-Glu. Both at low and high applied loads, this effect was likely due to the enhanced boundary lubrication enabled by HADN-Glu on the PCU surface. Moreover, HADN-Glu is highly biocompatible with chondrocyte cells, suggesting that this film will benefit the design of implants where lubrication is needed.
用于生物系统的生物材料，如聚碳酸酯聚氨酯（PCU）膝关节植入物和隐形眼镜，通常缺乏润滑性。这限制了它们与身体的整合，并阻碍了它们的功能。在此，我们提出了一种基于与多巴胺（HADN）和两性离子还原型谷胱甘肽（Glu）共轭的亲水性多糖透明质酸的纳米结构薄膜，它形成一种复合涂层（HADN-Glu）以增强软骨与PCU之间的润滑性。HADN通过透明质酸与多巴胺之间的碳二亚胺化学合成，并在温和的氧化条件下沉积在PCU表面。然后，将两性离子肽还原型谷胱甘肽与HADN进行生物共轭，形成润滑膜。基于X射线光电子能谱（XPS）、原子力显微镜（AFM）和润湿性的分析表明，HADN和Glu已成功接枝到PCU表面。摩擦系数（COF）、摩擦能量耗散和软骨粗糙度的测量表明，HADN-Glu的高润滑性有效地保护了软骨。在低和高施加载荷下，这种效果可能是由于HADN-Glu在PCU表面实现了增强的边界润滑。此外，HADN-Glu与软骨细胞具有高度生物相容性，这表明这种薄膜将有利于需要润滑的植入物的设计。
