Protective efficacy of attenuated Typhimurium strain expressing BLS, Omp19, PrpA, or SOD of in goats.

BACKGROUND

Attenuated strain can be used as a vector to transport immunogens to the host antigen-binding sites.

OBJECTIVES

The study aimed to determine the protective efficacy of attenuated strain expressing highly conserved immunogens in goats.

METHODS

Goats were vaccinated with vector expressing individually lipoprotein outer-membrane protein 19 (Omp19), lumazine synthase (BLS), proline racemase subunit A (PrpA), Cu/Zn superoxide dismutase (SOD) at 5 × 10⁹ CFU/mL and challenge of all groups was done at 6 weeks after vaccination.

RESULTS

Among these vaccines inoculated at 5 × 10⁹ CFU/mL in 1 mL, Omp19 or SOD showed significantly higher serum immunoglobulin G titers at (2, 4, and 6) weeks post-vaccination, compared to the vector control. Interferon-γ production in response to individual antigens was significantly higher in SOD, Omp19, PrpA, and BLS individual groups, compared to that in the vector control (all < 0.05). colonization rate at 8 weeks post-challenge showed that most vaccine-treated groups exhibited significantly increased protection by demonstrating reduced numbers of in tissues collected from vaccinated groups. Real-time polymerase chain reaction revealed that antigen expression levels were reduced in the spleen, kidney, and parotid lymph node of vaccinated goats, compared to the non-vaccinated goats. Besides, treatment with vaccine expressing individual antigens ameliorated brucellosis-related histopathological lesions.

CONCLUSIONS

These results delineated that BLS, Omp19, PrpA, and SOD proteins achieved a definite level of protection, indicating that Typhimurium successfully delivered antigens, and that individual vaccines could differentially elicit an antigen-specific immune response.