棕榈酸处理对 544 感染 RAW264.7 细胞和 BALB/c 小鼠的保护作用。

Protection of palmitic acid treatment in RAW264.7 cells and BALB/c mice during 544 infection.

机构信息

Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.

Bacterial Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.

出版信息

J Vet Sci. 2021 Mar;22(2):e18. doi: 10.4142/jvs.2021.22.e18.

DOI:10.4142/jvs.2021.22.e18

PMID:33774934

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8007444/

Abstract

BACKGROUND

We previously elucidated the protective mechanism of Korean red ginseng oil (RGO) against infection, and our phytochemical analysis revealed that palmitic acid (PA) was an abundant component of RGO. Consequently, we investigated the contribution of PA against .

OBJECTIVES

We aimed to investigate the efficacy of PA against infection using a murine cell line and a murine model.

METHODS

Cell viability, bactericidal, internalization, and intracellular replication, western blot, nitric oxide (NO), and superoxide (O₂⁻) analyses and flow cytometry were performed to determine the effects of PA on the progression of infection in macrophages. Flow cytometry for cytokine analysis of serum samples and bacterial counts from the spleens were performed to determine the effect of PA in a mouse model.

RESULTS

PA did not affect the growth of . PA treatment in macrophages did not change uptake but it did attenuate the intracellular survivability of . Incubation of cells with PA resulted in a modest increase in sirtuin 1 (SIRT1) expression. Compared to control cells, reduced nitrite accumulation, augmented O₂⁻, and enhanced pro-inflammatory cytokine production were observed in PA-treated -infected cells. Mice orally treated with PA displayed a decreased serum interleukin-10 level and enhanced bacterial resistance.

CONCLUSIONS

Our results suggest that PA participates in the control of within murine macrophages, and the study results confirm its efficacy against the infection. However, further investigations are encouraged to completely characterize the mechanisms involved in the inhibition of infection by fatty acids.

摘要

背景

我们之前阐明了高丽红参油（RGO）对感染的保护机制，我们的植物化学分析表明，棕榈酸（PA）是 RGO 的丰富成分。因此，我们研究了 PA 对感染的贡献。

目的

我们旨在使用鼠细胞系和鼠模型研究 PA 对感染的疗效。

方法

进行细胞活力、杀菌、内化和细胞内复制、western blot、一氧化氮（NO）和超氧化物（O₂⁻）分析以及流式细胞术，以确定 PA 对巨噬细胞中感染进展的影响。通过流式细胞术分析血清样本中的细胞因子和脾脏中的细菌计数，以确定 PA 在小鼠模型中的作用。

结果

PA 不影响的生长。PA 在巨噬细胞中的处理不会改变的摄取，但会减弱的细胞内存活率。PA 孵育可导致沉默信息调节因子 1（SIRT1）表达适度增加。与对照细胞相比，在 PA 处理的 -感染细胞中观察到亚硝酸盐积累减少、O₂⁻增加和促炎细胞因子产生增强。经 PA 口服治疗的小鼠显示血清白细胞介素-10 水平降低和细菌抵抗力增强。