Cong Shengnan, Li Jinlong, Zhang Jingjing, Feng Jingyi, Zhang Aixia, Pan Lianjun, Ma Jiehua
School of Nursing, Nanjing Medical University, Jiangsu, China.
Department of Laboratory Medicine, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing, China.
Front Cell Dev Biol. 2021 Mar 11;9:580834. doi: 10.3389/fcell.2021.580834. eCollection 2021.
Lubrication disorder is a common health issue that manifests as insufficient sexual arousal at the beginning of sex. It often causes physical and psychological distress. However, there are few studies on lubrication disorder, and the complexity of circular RNA (circRNA) and the related competing endogenous RNA (ceRNA) network in lubrication disorder is still poorly known. Therefore, this study aims to build a regulatory circRNA-micro (mi)RNA-mRNA network and explore potential molecular markers of lubrication disorder. In the study, 12 subjects were recruited, including 6 in the lubrication disorder group and 6 in the normal control group. RNA sequencing was exploited to identify the expression profiles of circRNA, miRNA and mRNA between two groups, and then to construct the circRNA-miRNA-mRNA networks. The enrichment analyses of the differentially expressed (DE)-mRNAs were examined via Gene Set Enrichment Analysis (GSEA). Furthermore, the expression level and interactions among circRNA, miRNA, and mRNA were validated using real-time quantitative polymerase chain reaction (RT-qPCR) and dual-luciferase reporter assays. In the results, 73 circRNAs, 287 miRNAs, and 354 target mRNAs were differentially expressed between two groups when taking | Log2 (fold change)| > 1 and -value < 0.05 as criteria, and then the results of GSEA revealed that DE-mRNAs were linked with "vascular smooth muscle contraction," "aldosterone regulated sodium reabsorption," "calcium signaling pathway," etc. 19 target relationships among 5 circRNAs, 4 miRNAs, and 7 mRNAs were found and constructed the ceRNA network. Among them, hsa-miR-212-5p and hsa-miR-874-3p were demonstrated to be related to the occurrence of lubrication disorder. Eventually, consistent with sequencing, RT-qPCR showed that hsa_circ_0026782 and were upregulated while hsa-miR-874-3p was downregulated, and dual-luciferase reporter assays confirmed the interactions among them. In summary, the findings indicate that the circRNA-miRNA-mRNA regulatory network is presented in lubrication disorder, and ulteriorly provide a deeper understanding of the specific regulatory mechanism of lubrication disorder from the perspective of the circRNA-miRNA-mRNA network.
润滑障碍是一种常见的健康问题，表现为性行为开始时性唤起不足。它常常导致身体和心理上的困扰。然而，关于润滑障碍的研究较少，环状RNA（circRNA）及相关竞争性内源性RNA（ceRNA）网络在润滑障碍中的复杂性仍知之甚少。因此，本研究旨在构建一个调控性的circRNA-微小（mi）RNA-信使核糖核酸（mRNA）网络，并探索润滑障碍的潜在分子标志物。在该研究中，招募了12名受试者，其中润滑障碍组6名，正常对照组6名。利用RNA测序来鉴定两组之间circRNA、miRNA和mRNA的表达谱，然后构建circRNA-miRNA-mRNA网络。通过基因集富集分析（GSEA）对差异表达（DE）-mRNA进行富集分析。此外，使用实时定量聚合酶链反应（RT-qPCR）和双荧光素酶报告基因检测来验证circRNA、miRNA和mRNA的表达水平及相互作用。结果显示，以| Log2（倍数变化）| > 1且P值 < 0.05为标准时，两组之间有73种circRNA、287种miRNA和354种靶mRNA差异表达，然后GSEA结果表明DE-mRNA与“血管平滑肌收缩”“醛固酮调节的钠重吸收”“钙信号通路”等有关。发现了5种circRNA、4种miRNA和7种mRNA之间的19种靶标关系并构建了ceRNA网络。其中，已证明hsa-miR-212-5p和hsa-miR-874-3p与润滑障碍的发生有关。最终，与测序结果一致，RT-qPCR显示hsa_circ_0026782和 上调，而hsa-miR-874-3p下调，双荧光素酶报告基因检测证实了它们之间的相互作用。总之，研究结果表明润滑障碍中存在circRNA-miRNA-mRNA调控网络，并进一步从circRNA-miRNA-mRNA网络的角度为深入理解润滑障碍的具体调控机制提供了依据。
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