Guo Jing, Zhao Jinhui, Liu Rui, Yu Jiaying, Zhang Mingjia, Wang Hanming, Liu Liyan
Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, 157 Baojian Road, Nangang District, Harbin, 150086, People's Republic of China.
The Department of Clinical Nutrition, Southern University of Science and Technology Hospital, Shenzhen, People's Republic of China.
Metabolomics. 2021 Mar 31;17(4):38. doi: 10.1007/s11306-021-01788-1.
Nephrotic syndrome (NS) is a common pediatric urinary system disease. The aim in this work was to investigate the changes in pediatric NS-related metabolites through serum metabolomics, and explore the new potential metabolites and differential metabolic pathways.
Serum samples from 40 pediatric patients with nephrotic syndrome and 40 healthy controls were collected. The targeted and non-targeted metabolomics analyses were performed to determine the metabolic changes in pediatric NS. Based on multivariate statistical analysis and the regression model, the serum potential metabolites were screened and different metabolic pathways were explored.
39 differential metabolites in pediatric NS were obtained based on the metabolomics analysis. 12 differential metabolites (serine, C18: 2 (EFA), C18: 2 (FFA), Isonuatigenin 3- [rhamnosyl- (1- > 2) -glucoside], C18: 4 (EFA), C18: 4 (FFA), caprylic acid, citric acid, methylmalonic acid, caproic acid, canavalioside and uroporphyrin were identified to establish the diagnostic model for pediatric NS. Five metabolic pathways including TCA cycle, amino acid metabolism, bile acid biosynthesis, linoleate metabolism and glyoxylate and dicarboxylate metabolism were the key differential metabolic pathways.
These data elucidated the metabolic alterations associated with pediatric NS and suggested a new diagnosis model for monitoring pediatric NS. The current study provides the useful information to bridge the gaps in our understanding of the metabolic alterations associated with pediatric NS and might facilitate the characterization of pediatric NS patients by performing serum metabolomics.
肾病综合征（NS）是一种常见的儿科泌尿系统疾病。本研究旨在通过血清代谢组学研究儿科肾病综合征相关代谢物的变化，并探索新的潜在代谢物和差异代谢途径。
收集40例儿科肾病综合征患者和40例健康对照的血清样本。进行靶向和非靶向代谢组学分析以确定儿科肾病综合征的代谢变化。基于多变量统计分析和回归模型，筛选血清潜在代谢物并探索不同的代谢途径。
基于代谢组学分析获得了39种儿科肾病综合征差异代谢物。鉴定出12种差异代谢物（丝氨酸、C18:2（必需脂肪酸）、C18:2（游离脂肪酸）、异欧前胡素3 - [鼠李糖基 - (1->2)-葡萄糖苷]、C18:4（必需脂肪酸）、C18:4（游离脂肪酸）、辛酸、柠檬酸、甲基丙二酸、己酸、刀豆球蛋白A糖苷和尿卟啉）以建立儿科肾病综合征的诊断模型。包括三羧酸循环、氨基酸代谢、胆汁酸生物合成、亚油酸代谢和乙醛酸及二羧酸代谢在内的五条代谢途径是关键的差异代谢途径。
这些数据阐明了与儿科肾病综合征相关的代谢改变，并提出了一种监测儿科肾病综合征的新诊断模型。本研究提供了有用信息，以填补我们对与儿科肾病综合征相关代谢改变理解上的空白，并可能通过进行血清代谢组学促进儿科肾病综合征患者的特征化。
