Molecular and Applied Mycology and Plant Pathology Laboratory, Center of Advanced Study, Department of Botany, University of Calcutta, Kolkata, India.
J Pharm Pharmacol. 2021 Mar 1;73(1):70-81. doi: 10.1093/jpp/rgaa020.
Calocybe indica is a famous nutritious food in Asian countries and one of the most widely cultivated mushrooms in the world. Here, we have isolated crude polysaccharides from this mushroom, characterized it and investigated its antioxidant and immunostimulatory potential.
The polysaccharide was chemically characterized by spectrophotometry, FTIR and high-performance thin layer chromatography and tested its antioxidant potential by in vitro assays. Immunomodulatory activity and its underlying signalling process were ascertained in RAW 264.7 cells.
The polysaccharide consisted of D-glucose (β-linked sugars), D-mannose and D-galactose, where backbone was organized in random coil structure. Preliminary investigation of the bioactivity of the polysaccharide revealed its antioxidant potential. The polysaccharide could noticeably induce phagocytic activity and production of immune mediators in macrophage cells. The polysaccharide was found to enhance the expression of pro-inflammatory cytokines and activate NF-κB signalling pathway by overexpressing MyD88, Iκ-Bα and NF-κB. Further studies indicated the polysaccharide binds to the toll-like receptor 4 to manifest its immunostimulatory activity in macrophage cells.
Our findings indicate potential therapeutic properties of the crude polysaccharide of C. indica which might provide the means to treat various radical induced and immunodeficiency disorders in the days to come.
鸡枞菌是亚洲国家著名的营养食品，也是世界上栽培最广泛的蘑菇之一。在这里，我们从这种蘑菇中分离出粗多糖，对其进行了表征，并研究了其抗氧化和免疫刺激潜力。
采用分光光度法、傅里叶变换红外光谱和高效薄层色谱对多糖进行化学表征，并通过体外试验测试其抗氧化潜力。在 RAW 264.7 细胞中确定免疫调节活性及其潜在的信号转导过程。
多糖由 D-葡萄糖（β-连接糖）、D-甘露糖和 D-半乳糖组成，其主链呈无规卷曲结构。对多糖生物活性的初步研究表明其具有抗氧化潜力。多糖能明显诱导巨噬细胞的吞噬活性和免疫介质的产生。研究发现，多糖通过过度表达 MyD88、Iκ-Bα 和 NF-κB，增强促炎细胞因子的表达并激活 NF-κB 信号通路。进一步的研究表明，多糖与 Toll 样受体 4 结合，在巨噬细胞中表现出免疫刺激活性。
我们的研究结果表明，鸡枞菌粗多糖具有潜在的治疗特性，这可能为未来治疗各种自由基诱导和免疫缺陷疾病提供方法。
