γ-氨基丁酸激动剂诱导的培养小脑颗粒细胞超微结构改变仅限于早期发育阶段。

Gamma-aminobutyric acid agonist-induced alterations in the ultrastructure of cultured cerebellar granule cells is restricted to early development.

作者信息

Hansen G H, Belhage B, Schousboe A, Meier E

机构信息

Department of Biochemistry A, The Panum Institute, University of Copenhagen, Denmark.

出版信息

J Neurochem. 1988 Jul;51(1):243-5. doi: 10.1111/j.1471-4159.1988.tb04862.x.

DOI:10.1111/j.1471-4159.1988.tb04862.x

PMID:3379406

Abstract

The effect of 4,5,6,7-tetrahydroisoxazolo[5,4-c]-pyridin-3-ol (THIP) on the ultrastructural composition of cultured cerebellar granule cells was investigated during development by quantitative electron microscopy (morphometric analysis). Granule cells were exposed to THIP (150 microM) for 6 h after 7 and 14 days, respectively, in culture. THIP treatment of 7-day-old cultures led to a statistically significant increase in the cytoplasmic density of rough endoplasmic reticulum, Golgi apparatus, vesicles, and coated vesicles, whereas no significant increase in the cytoplasmic density of these organelles was observed in 14-day-old cultures exposed to THIP for 6 h. These findings show that the effect of THIP on the ultrastructural composition of cultured cerebellar granule cells is restricted to early development.

摘要

通过定量电子显微镜（形态计量分析）研究了4,5,6,7-四氢异恶唑并[5,4-c]-吡啶-3-醇（THIP）在发育过程中对培养的小脑颗粒细胞超微结构组成的影响。在培养7天和14天后，分别将颗粒细胞暴露于THIP（150微摩尔）6小时。用THIP处理7天大的培养物导致粗面内质网、高尔基体、囊泡和被膜小泡的细胞质密度在统计学上显著增加，而在暴露于THIP 6小时的14天大的培养物中，未观察到这些细胞器的细胞质密度有显著增加。这些发现表明，THIP对培养的小脑颗粒细胞超微结构组成的影响仅限于早期发育阶段。