在大鼠排卵过程中组织因子和组织因子途径抑制剂的表达：与卵巢过度刺激综合征的相关性。

Expression of tissue factor and tissue factor pathway inhibitors during ovulation in rats: a relevance to the ovarian hyperstimulation syndrome.

机构信息

Animal Facility of Aging Science, Korea Basic Science Institute, Gwangju, 61186, Republic of Korea.

School of Biological Sciences and Biotechnology, Faculty of Life Science, Chonnam National University, Gwangju, 61186, Republic of Korea.

出版信息

Reprod Biol Endocrinol. 2021 Apr 1;19(1):52. doi: 10.1186/s12958-021-00708-1.

DOI:10.1186/s12958-021-00708-1

PMID:33794911

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8017805/

Abstract

BACKGROUND

Blood coagulation has been associated with ovulation and female infertility. In this study, the expression of the tissue factor system was examined during ovulation in immature rats; the correlation between tissue factor and ovarian hyperstimulation syndrome (OHSS) was evaluated both in rats and human follicular fluids.

METHODS

Ovaries were obtained at various times after human chorionic gonadotropin (hCG) injection to investigate the expression of tissue factor system. Expression levels of ovarian tissue factor, tissue factor pathway inhibitor (Tfpi)-1 and Tfpi-2 genes and proteins were determined by real-time quantitative polymerase chain reaction (qPCR), and Western blot and immunofluorescence analyses, respectively. Expression levels of tissue factor system were also investigated in ovaries of OHSS-induced rats and in follicular fluid of infertile women.

RESULTS

The expression of tissue factor in the preovulatory follicles was stimulated by hCG, reaching a maximum at 6 h. Tissue factor was expressed in the oocytes and the preovulatory follicles. Tfpi-2 mRNA levels were mainly increased by hCG in the granulosa cells whereas the mRNA levels of Tfpi-1 were decreased by hCG. Human CG-stimulated tissue factor expression was inhibited by the progesterone receptor antagonist. The increase in Tfpi-2 expression by hCG was decreased by the proliferator-activated receptor γ (PPARγ) antagonist. Decreased expression of the tissue factor was detected in OHSS-induced rats. Interestingly, the tissue factor concentrations in the follicular fluids of women undergoing in vitro fertilization were correlated with pregnancy but not with OHSS.

CONCLUSIONS

Collectively, the results indicate that tissue factor and Tfpi-2 expression is stimulated during the ovulatory process in rats; moreover, a correlation exists between the levels of tissue factor and OHSS in rats but not in humans.

摘要

背景

凝血与排卵和女性不孕有关。在这项研究中，研究了组织因子系统在未成熟大鼠排卵过程中的表达；评估了组织因子与卵巢过度刺激综合征（OHSS）在大鼠和人卵泡液中的相关性。

方法

在人绒毛膜促性腺激素（hCG）注射后不同时间获取卵巢，以研究组织因子系统的表达。通过实时定量聚合酶链反应（qPCR）、Western blot 和免疫荧光分析分别测定卵巢组织因子、组织因子途径抑制剂（Tfpi）-1 和 Tfpi-2 基因和蛋白的表达水平。还研究了 OHSS 诱导的大鼠卵巢和不孕妇女卵泡液中组织因子系统的表达。

结果

hCG 刺激前排卵泡中组织因子的表达，在 6 小时达到高峰。组织因子在卵母细胞和前排卵泡中表达。Tfpi-2 mRNA 水平主要由 hCG 在颗粒细胞中增加，而 Tfpi-1 的 mRNA 水平则由 hCG 降低。孕激素受体拮抗剂抑制 hCG 刺激的组织因子表达。hCG 增加 Tfpi-2 的表达被过氧化物酶体增殖物激活受体 γ（PPARγ）拮抗剂减少。在 OHSS 诱导的大鼠中检测到组织因子表达减少。有趣的是，接受体外受精的妇女卵泡液中的组织因子浓度与妊娠相关，但与 OHSS 无关。