Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong S.A.R., China.
STAR Protoc. 2021 Mar 16;2(2):100393. doi: 10.1016/j.xpro.2021.100393. eCollection 2021 Jun 18.
Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here, we detail the preparations, surgical procedures, ultrasound stimulation process, and simultaneous electromyogram (EMG) measurement necessary for successful sonogenetic stimulation in mice. For complete details on the use and execution of this protocol, please refer to Qiu et al. (2020).
通过靶向超声干预来操纵特定的神经活动是深入了解大脑功能和治疗大脑疾病的一种有力方法。声遗传学技术可用于控制经超声敏感离子通道基因修饰的细胞。在此，我们详细介绍了在小鼠中成功进行声遗传学刺激所需的准备、手术程序、超声刺激过程和同时进行的肌电图（EMG）测量。如需了解此方案的使用和执行的完整详细信息，请参见 Qiu 等人（2020 年）。
