Chaisiri Chingchai, Liu Xiangyu, Lin Yang, Fu Yanping, Zhu Fuxing, Luo Chaoxi
Key Lab of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
Key Lab of Crop Disease Monitoring and Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.
Biology (Basel). 2021 Mar 1;10(3):179. doi: 10.3390/biology10030179.
is considered one of the most important causal agents of many plant diseases, with a broad host range worldwide. In this study, multiple sequences of ribosomal internal transcribed spacer region (ITS), translation elongation factor 1-α gene (), beta-tubulin gene (), calmodulin gene (), and histone-3 gene () were used for multi-locus phylogenetic analysis. For phylogenetic analysis, maximum likelihood (ML), maximum parsimony (MP), and Bayesian inferred (BI) approaches were performed to investigate relationships of with closely related species. The results strongly support that the species falls into a monophyletic lineage, with the characteristics of a species complex. Phylogenetic informativeness (PI) analysis showed that clear boundaries could be proposed by using , whereas ITS showed an ineffective reconstruction and, thus, was unsuitable for speciating boundaries for species. A combined dataset of , , , and showed strong resolution for species, providing insights for the complex. Accordingly, besides , , , , , , , , , and , which have already been previously considered the synonymous species of , another three species, , and , were further revealed to be synonyms of in this study. In order to demonstrate the genetic diversity of species in China, 138 isolates were randomly selected from previous studies in 16 provinces. These isolates were obtained from different major plant species from 2006 to 2020. The genetic distance was estimated with phylogenetic analysis and haplotype networks, and it was revealed that two major haplotypes existed in the Chinese populations of . The haplotype networks were widely dispersed and not uniquely correlated to specific populations. Overall, our analyses evaluated the phylogenetic identification for species and demonstrated the population diversity of in China.
被认为是许多植物病害最重要的致病因子之一，在全球范围内具有广泛的寄主范围。在本研究中，核糖体内部转录间隔区（ITS）、翻译延伸因子1-α基因（）、β-微管蛋白基因（）、钙调蛋白基因（）和组蛋白-3基因（）的多个序列被用于多位点系统发育分析。对于系统发育分析，采用最大似然法（ML）、最大简约法（MP）和贝叶斯推断法（BI）来研究该物种与近缘物种的关系。结果有力地支持该物种属于一个单系谱系，具有物种复合体的特征。系统发育信息性（PI）分析表明，使用基因可以提出清晰的界限，而ITS显示出无效的重建，因此不适合用于该物种的物种界限划分。基因、、和的组合数据集对该物种具有很强的分辨率，为该复合体提供了见解。因此，除了之前已被认为是该物种同义种的、、、、、、、、和之外，本研究还进一步揭示另外三个物种、和也是该物种的同义词。为了展示中国该物种的遗传多样性，从之前在16个省份的研究中随机选取了138个该物种的分离株。这些分离株是在2006年至2020年期间从不同的主要植物物种中获得的。通过系统发育分析和单倍型网络估计了遗传距离，结果表明中国该物种群体中存在两种主要单倍型。单倍型网络分布广泛，与特定群体没有唯一的相关性。总体而言，我们的分析评估了该物种的系统发育鉴定，并展示了中国该物种的群体多样性。
