Institute of Food Science, BOKU-University of Natural Resources and Life Sciences Vienna, 1190 Vienna, Austria.
Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zürich, 8057 Zurich, Switzerland.
Toxins (Basel). 2021 Mar 24;13(4):234. doi: 10.3390/toxins13040234.
belongs to the group that also comprises the foodborne pathogen sensu stricto, causing anthrax, as well as the biopesticide . The first was isolated in the context of a severe food poisoning outbreak leading to fatal cases of diarrheal disease. Subsequent characterization of the outbreak strain led to the conclusion that this strain was highly cytotoxic and eventually resulted in the description of a novel species, whose name reflects the observed toxicity: . However, only a few isolates of this species have been characterized with regard to their cytotoxic potential and the role of as a causative agent of food poisoning remains largely unclear. Hence, the aim of this study was to gain further insights into the toxicity of . To this end, 19 isolates were obtained from mashed potato powders and characterized by toxin gene profiling and Vero cell cytotoxicity assays. All isolates harbored the (cytotoxin K1) gene and species-specific variants of the (non-hemolytic enterotoxin) gene. The isolates exhibited low or no toxicity towards Vero cells. Thus, this study indicates that the cytotoxic potential of may be potentially lower than initially assumed.
属于还包括食源性病原体严格意义上的炭疽以及生物农药的组。第一个是在导致致命腹泻病病例的严重食物中毒爆发的背景下分离出来的。随后对爆发菌株的特征描述得出结论，该菌株具有高度细胞毒性，最终导致了一种新型物种的描述，其名称反映了观察到的毒性：。然而，只有少数该物种的分离株在细胞毒性潜力方面得到了表征，并且作为食物中毒的病原体的作用在很大程度上仍不清楚。因此，本研究的目的是进一步了解的毒性。为此，从土豆泥粉中获得了 19 个分离株，并通过毒素基因谱分析和 Vero 细胞细胞毒性测定进行了表征。所有分离株均携带（细胞毒素 K1）基因和（非溶血肠毒素）基因的种特异性变体。分离株对 Vero 细胞的毒性较低或没有。因此，本研究表明，的细胞毒性潜力可能比最初假设的要低。
