State Key Laboratory of Animal Nutrition, Beijing Engineering Technology, Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian, Beijing 100193, China.
The Cell Wall Utilization and Biology Laboratory, USDA Agricultural Research Service, US Dairy Forage Research Center, Madison, WI 53706, USA.
Genes (Basel). 2021 Mar 16;12(3):424. doi: 10.3390/genes12030424.
This study aimed to investigate the changes in abomasum transcriptome and the associated microbial community structure in young calves with artificially dosed, adult rumen contents. Eight young bull calves were randomly dosed with freshly extracted rumen contents from an adult cow (high efficiency (HE), = 4), or sterilized rumen content (Con, = 4). The dosing was administered within 3 days of birth, then at 2, 4, and 6 weeks following the initial dosing. Abomasum tissues were collected immediately after sacrifice at 8 weeks of age. Five genera (; < 0.05) showed significant difference in abundance between the treatments. A total of 975 differentially expressed genes were identified ( < 0.05, fold-change > 1.5, mean read-counts > 5). Pathway analysis indicated that up-regulated genes were involved in immune system process and defense response to virus, while the down-regulated genes involved in ion transport, ATP biosynthetic process, and mitochondrial electron transport. Positive correlation (r > 0.7, < 0.05) was observed between gene and , which was significantly higher in the HE group. had a reported role in the immune system process. In conclusion, the dosing of adult rumen contents to calves can alter not only the composition of active microorganisms in the abomasum but also the molecular mechanisms in the abomasum tissue, including reduced protease secretion and decreased hydrochloric acid secretion.
本研究旨在探究人工投喂成年牛瘤胃液对犊牛皱胃转录组和相关微生物群落结构的变化。8 头公牛犊牛随机投喂取自成年牛的新鲜提取瘤胃液（高效组，n = 4）或灭菌瘤胃液（对照组，n = 4）。犊牛在出生后 3 天内进行首次投喂，然后在首次投喂后 2、4 和 6 周时进行。在 8 周龄处死时立即采集皱胃组织。处理组间有 5 个属（ < 0.05）的丰度有显著差异。共鉴定出 975 个差异表达基因（ < 0.05，fold-change > 1.5，mean read-counts > 5）。通路分析表明，上调基因参与免疫系统过程和对病毒的防御反应，而下调基因参与离子转运、ATP 生物合成过程和线粒体电子传递。基因和 之间存在正相关（r > 0.7， < 0.05），高效组中相关性更高。 被报道在免疫系统过程中发挥作用。综上所述，向犊牛投喂成年牛瘤胃液不仅可以改变皱胃中活跃微生物的组成，还可以改变皱胃组织中的分子机制，包括蛋白酶分泌减少和盐酸分泌减少。
