一种新型的基于罗丹明的荧光探针，用于快速和选择性检测 BSA、水、牛奶、白菜、萝卜、苹果和梨中的半胱氨酸。
A novel rosamine-based fluorescent probe for the rapid and selective detection of cysteine in BSA, water, milk, cabbage, radish, apple, and pear.
机构信息
Tianjin Key Laboratory of Structure and Performance for Functional Molecules, College of Chemistry, Tianjin Normal University, Tianjin 300387, China.
Tianjin Key Laboratory of Structure and Performance for Functional Molecules, College of Chemistry, Tianjin Normal University, Tianjin 300387, China.
出版信息
Food Chem. 2021 Sep 15;356:129658. doi: 10.1016/j.foodchem.2021.129658. Epub 2021 Mar 23.
A novel fluorescent probe (RA), based on the rosamine skeleton bearing acrylate group, has been reasonably designed and prepared, which employed an addition-cyclization-elimination sequence reaction mechanism to detect cysteine. RA displayed rapid response to cysteine within 1.5 min, and exhibited satisfactory selectivity for cysteine over HS, glutathione (Glu), and homocysteine (Hcy), due to the formation of seven-membered lactam favored kinetically. Fluorescence ratio was utilized to detect cysteine from 6.0 to 20.0 μM with a detection limit of 0.29 μM. More, RA was used to monitor cysteine in BSA, water, milk, milk powder, cabbage, radish, apple, and pear.
一种基于带有丙烯酰胺基的若丹明骨架的新型荧光探针（RA）已被合理设计和制备，该探针采用加成-环化-消除序列反应机制来检测半胱氨酸。RA 与半胱氨酸的反应在 1.5 分钟内迅速发生，并且由于动力学上有利的七元内酰胺的形成，对半胱氨酸具有令人满意的选择性，优于 HS、谷胱甘肽 (Glu) 和同型半胱氨酸 (Hcy)。荧光比用于检测 6.0 至 20.0 μM 范围内的半胱氨酸，检测限为 0.29 μM。此外，RA 还用于监测 BSA、水、牛奶、奶粉、白菜、萝卜、苹果和梨中的半胱氨酸。
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