Hikosaka Masashi, Iwahashi Fukumatsu, Yamato Seiji
Health & Crop Science Research Laboratory, Sumitomo Chemical Co., Ltd, Takarazuka, Hyogo 665-8555, Japan.
Pestic Biochem Physiol. 2021 May;174:104827. doi: 10.1016/j.pestbp.2021.104827. Epub 2021 Mar 10.
Despite the increase in pressure for reducing the use of chemical pesticides in agriculture, herbicides remain one of the efficient tools for augmenting food production. Various herbicide-resistant weeds against most herbicidal modes of action (MoA) are emerging worldwide, and therefore, the necessity of developing herbicides with novel MoA is increasing. Toward this, rigid methods of determining MOA that can be applied for various weeds species are required. Despite the existence of weed species with resistance to acetolactate synthase (ALS) inhibitors, inhibition of ALS remains one of the most widely used herbicidal MoAs containing more than 50 commercial active ingredients. Here, we aimed to identify marker metabolites that are indicative of ALS inhibition. We performed non-targeted and targeted metabolomics using ALS inhibitor-treated Schoenoplectus juncoides. We identified internal metabolite markers for ALS inhibition, with excellent selectivity for ALS inhibitors and herbicides with different MOAs in various weed species. These markers will enable us to evaluate ALS inhibitory activity of chemicals in vivo in a wide variety of weed species.
尽管在农业中减少化学农药使用的压力不断增加，但除草剂仍然是提高粮食产量的有效工具之一。世界各地正在出现针对大多数除草作用模式（MoA）的各种抗除草剂杂草，因此，开发具有新型作用模式的除草剂的必要性日益增加。为此，需要能够应用于各种杂草物种的确定作用模式的严格方法。尽管存在对乙酰乳酸合酶（ALS）抑制剂具有抗性的杂草物种，但抑制ALS仍然是最广泛使用的除草作用模式之一，包含50多种商业活性成分。在这里，我们旨在鉴定指示ALS抑制的标记代谢物。我们使用ALS抑制剂处理的黑麦草进行了非靶向和靶向代谢组学研究。我们确定了ALS抑制的内部代谢物标记，对各种杂草物种中的ALS抑制剂和具有不同作用模式的除草剂具有出色的选择性。这些标记将使我们能够在多种杂草物种中体内评估化学品的ALS抑制活性。
