Borglund M, Akesson B
Department of Clinical Chemistry, University Hospital, Lund, Sweden.
Int J Vitam Nutr Res. 1988;58(1):97-102.
The influence of an increased intake of selenium on the distribution of this element among plasma proteins was studied. 200 micrograms of yeast selenium was given to healthy subjects daily for 8 weeks, and then the subjects refrained from selenium supplementation for 16 weeks. Plasma selenium increased almost two-fold during supplementation, and most of the increase occurred during the first 4-week-period. Plasma glutathione peroxidase activity increased only marginally. At all times studied, most of the selenium in plasma was located in proteins, migrating close to immunoglobulin G on gel filtration. The selenium content of this fraction was only moderately increased after supplementation for 8 weeks, and instead more marked increases occurred in the regions for high-molecular-weight proteins and albumin. This change implied that the distribution of selenium approached that of total protein, and we therefore conclude that most of the increase in plasma selenium occurred via unspecific incorporation of selenium into a wide variety of proteins. Sixteen weeks after the end of supplementation the selenium distribution had essentially returned to that before supplementation.
研究了增加硒摄入量对该元素在血浆蛋白中分布的影响。每天给健康受试者服用200微克酵母硒，持续8周，然后受试者停止补充硒16周。补充期间血浆硒增加了近两倍，且大部分增加发生在最初的4周内。血浆谷胱甘肽过氧化物酶活性仅略有增加。在所有研究时间点，血浆中的大部分硒都存在于蛋白质中，在凝胶过滤时靠近免疫球蛋白G迁移。补充8周后，该部分的硒含量仅适度增加，而高分子量蛋白质和白蛋白区域的增加更为明显。这种变化意味着硒的分布接近总蛋白的分布，因此我们得出结论，血浆硒的增加大部分是通过硒非特异性地掺入多种蛋白质中实现的。补充结束16周后，硒的分布基本恢复到补充前的水平。
