用于活细胞中有效半胱氨酸成像的具有大斯托克斯位移的探针。
Probe with large Stokes shift for effective cysteine imaging in living cells.
作者信息
Zou Fengxia, Wang Chun, Song Wenwu, Shen Lujie, Xu Runsheng, Wang Miao, Wang Minmin, Sun Tongming, Wang Jin
机构信息
School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, PR China.
School of Textile and Clothing, Nantong University, Nantong 226001, PR China; Nantong Key Laboratory of Intelligent and New Energy Materials, Nantong 226001, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Aug 5;257:119775. doi: 10.1016/j.saa.2021.119775. Epub 2021 Apr 1.
A new fluorescence probe L, which featured with a large Stokes shift (216 nm), was designed for sensitive detection of cysteine (Cys) and a potential sensing mechanism derived from excited state intramolecular proton transfer (ESIPT) was proposed. More importantly, probe L exhibits higher selective to Cys than other amino acid due to its specific cyclization between acrylate group and Cys. Meanwhile, the probe L shows a low detection limit of 8.82 × 10 M, which is enough for detecting Cys in organisms. Furthermore, this probe displays high biocompatibility and can image Cys in living cells.
设计了一种具有大斯托克斯位移（216 nm）的新型荧光探针L，用于灵敏检测半胱氨酸（Cys），并提出了一种基于激发态分子内质子转移（ESIPT）的潜在传感机制。更重要的是，由于探针L的丙烯酸酯基团与Cys之间存在特异性环化作用，其对Cys的选择性高于其他氨基酸。同时，探针L的检测限低至8.82×10⁻⁸ M，足以检测生物体内的Cys。此外，该探针具有高生物相容性，可对活细胞中的Cys进行成像。
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