Obode Okukwe Christa, Adebayo Abiodun Humphrey, Li Chunyang
Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Ogun State Nigeria.
Nutrition and Toxicology Division, Food Technology Department, Federal Institute of Industrial Research Oshodi, Lagos, Nigeria.
Toxicol Res. 2020 Jul 6;37(2):183-195. doi: 10.1007/s43188-020-00052-3. eCollection 2021 Apr.
In this study, we investigated the phytochemical and toxicological impacts of the ethanol leaf extract of in albino rats. Preliminary phytochemical analysis was done using established methods. Acute and 28-day sub-chronic toxicity evaluations of the extract were carried out in wistar rats. Five groups made up of 8 rats per group were used and treated with 500, 1000, 1500 and 2000 mg/kg b.wt. respectively; while the control group received 1 ml of 10% Tween-20 solution. Phytochemicals detected include tannins, saponins, flavonoids, alkaloids, anthocyanin, quinones, terpenoids and steroids. There were no signs of toxicity in the acute toxicity study neither was any death recorded. Weight loss and death were recorded after 18 days of treatment in groups treated with 1500 and 2000 mg/kg b.wt. No significant alteration of haematological parameters was observed, however increased WBC count was recorded. Liver and kidney function parameters were significantly ( < 0.05) reduced, while significant ( < 0.05) increase in triglycerides with lowered cholesterol was seen when compared with the control group. Histological evaluations of the liver, kidney, heart and testicular tissues revealed normal sections in majority of the animals; however, mild vascular congestions were observed at random doses (liver and heart at 1500 mg/kg b.wt.; kidney at 500 mg/kg b.wt.). Our study shows that ethanol leaf extract of would probably not cause significant toxic effects as indicated by haematological and biochemical parameters. However, there may be need to apply caution in its use at high doses given that death occurred at 1500 mg/kg b.wt. during the repeat-dose toxicity study.
在本研究中，我们调查了[植物名称]乙醇叶提取物对白化大鼠的植物化学和毒理学影响。采用既定方法进行了初步植物化学分析。在Wistar大鼠中对该提取物进行了急性和28天亚慢性毒性评估。使用五组，每组8只大鼠，分别用500、1000、1500和2000mg/kg体重进行处理；而对照组接受1ml 10%吐温-20溶液。检测到的植物化学物质包括单宁、皂苷、黄酮类化合物、生物碱、花青素、醌类、萜类化合物和甾体。急性毒性研究中未出现毒性迹象，也未记录到任何死亡情况。在接受1500和2000mg/kg体重处理的组中，处理18天后记录到体重减轻和死亡情况。未观察到血液学参数有显著改变，但白细胞计数有所增加。与对照组相比，肝功能和肾功能参数显著（P<0.05）降低，同时甘油三酯显著（P<0.05）升高而胆固醇降低。对肝脏、肾脏、心脏和睾丸组织的组织学评估显示，大多数动物的切片正常；然而，在随机剂量下观察到轻度血管充血（肝脏和心脏在1500mg/kg体重时；肾脏在500mg/kg体重时）。我们的研究表明，[植物名称]乙醇叶提取物可能不会像血液学和生化参数所表明的那样引起显著的毒性作用。然而，鉴于在重复剂量毒性研究中1500mg/kg体重时出现了死亡情况，在高剂量使用时可能需要谨慎。
