硫酸化胆囊收缩素八肽（CCK8）未能调节大鼠纹状体中的基础或多巴胺刺激的腺苷酸环化酶活性。

Sulfated cholecystokinin octapeptide (CCK8) failed to modulate basal or dopamine-stimulated adenylate cyclase activity in the rat striatum.

Morency M A, Ross G M, Kajiura J S, Mishra R K

Department of Psychiatry, McMaster University, Hamilton, Ontario, Canada.

Prog Neuropsychopharmacol Biol Psychiatry. 1988;12(2-3):331-6. doi: 10.1016/0278-5846(88)90052-8.

Abstract

In view of previously demonstrated modulatory effects of CCK8 on DA-sensitive adenylate cyclase activity in the nucleus accumbens, we examined the effects of this neuropeptide in the striatum. 2. Adenylate cyclase activity was measured by conversion of alpha-[32P]ATP into [32P]cAMP. 3. CCK8, when added to the adenylate cyclase assay in concentrations up to 100 microM, failed to significantly alter, either positively or negatively, basal or DA-stimulated (30 or 100 microM) adenylate cyclase activity. Similar results were obtained in the presence of various peptidase inhibitors. 4. Under the assay conditions employed in these experiments, it would appear that there is no effect of CCK8 on DA-sensitive adenylate cyclase in the striatum.

摘要

鉴于先前已证明胆囊收缩素八肽（CCK8）对伏隔核中对多巴胺（DA）敏感的腺苷酸环化酶活性具有调节作用，我们研究了这种神经肽在纹状体中的作用。2. 通过将α-[32P]ATP转化为[32P]cAMP来测量腺苷酸环化酶活性。3. 当以高达100微摩尔的浓度添加到腺苷酸环化酶测定中时，CCK8未能显著改变基础或DA刺激（30或100微摩尔）的腺苷酸环化酶活性，无论是正向还是负向。在存在各种肽酶抑制剂的情况下也获得了类似的结果。4. 在这些实验所采用的测定条件下，似乎CCK8对纹状体中对DA敏感的腺苷酸环化酶没有作用。